Overview

  • Product name
    Anti-BRN3B / POU4F2 antibody
    See all BRN3B / POU4F2 primary antibodies
  • Description
    Goat polyclonal to BRN3B / POU4F2
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control
    • WB: Rat pup PND2-6 – minimal cortex brain lysate. IHC-P: Mouse testis tissue. ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab235268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 43 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
  • Tissue specificity
    Brain. Seems to be specific to the retina.
  • Sequence similarities
    Belongs to the POU transcription factor family. Class-4 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization
    Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain specific homeobox/POU domain protein 3B antibody
    • Brain-3B antibody
    • Brain-specific homeobox/POU domain protein 3B antibody
    • Brn-3B antibody
    • BRN3.2 antibody
    • BRN3B antibody
    • Brn3b POU domain transcription factor antibody
    • class 4 antibody
    • PO4F2_HUMAN antibody
    • POU class 4 homeobox 2 antibody
    • POU domain antibody
    • POU domain class 4 transcription factor 2 antibody
    • POU domain protein antibody
    • POU4F2 antibody
    • transcription factor 2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BRN3B / POU4F2 with ab235268 at 1/100 dilution (30 minutes at RT), followed by Donkey anti-Goat (HRP) (4µl/ml, 20 minutes at RT). Primary staining is DAB. Counterstained with hematoxylin.
    Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.

  • Anti-BRN3B / POU4F2 antibody (ab235268) at 1/1000 dilution + Rat pup PND2-6 – minimal cortex brain lysate at 35 µg

    Predicted band size: 43 kDa


    Exposure time: 12 seconds
  • Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized Post-natal rat pup (PND1) heterogeneous brain cells labeling BRN3B / POU4F2 with ab235268 at 5 µg/ml (Overnight at 4°C), followed by a Donkey anti-Goat IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 µg/ml (1 hour at RT) (green).
    Expected localization: Nuclear, nuclear speckle. The nuclear counterstain is DAPI (blue).
    Panel A: DAPI.
    Panel B: ab235268 + secondary antibody.
    Panel C: Merge of panel A and B.
    Panel D: Secondary only.

References

ab235268 has not yet been referenced specifically in any publications.

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