Product nameAnti-BRN3B / POU4F2 antibody
See all BRN3B / POU4F2 primary antibodies
DescriptionGoat polyclonal to BRN3B / POU4F2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: Rat pup PND2-6 – minimal cortex brain lysate. IHC-P: Mouse testis tissue. ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 43 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionTranscription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
Tissue specificityBrain. Seems to be specific to the retina.
Sequence similaritiesBelongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Cellular localizationNucleus speckle.
- Information by UniProt
- Brain specific homeobox/POU domain protein 3B antibody
- Brain-3B antibody
- Brain-specific homeobox/POU domain protein 3B antibody
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BRN3B / POU4F2 with ab235268 at 1/100 dilution (30 minutes at RT), followed by Donkey anti-Goat (HRP) (4µl/ml, 20 minutes at RT). Primary staining is DAB. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.
Anti-BRN3B / POU4F2 antibody (ab235268) at 1/1000 dilution + Rat pup PND2-6 – minimal cortex brain lysate at 35 µg
Predicted band size: 43 kDa
Exposure time: 12 seconds
Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized Post-natal rat pup (PND1) heterogeneous brain cells labeling BRN3B / POU4F2 with ab235268 at 5 µg/ml (Overnight at 4°C), followed by a Donkey anti-Goat IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 µg/ml (1 hour at RT) (green).
Expected localization: Nuclear, nuclear speckle. The nuclear counterstain is DAPI (blue).
Panel A: DAPI.
Panel B: ab235268 + secondary antibody.
Panel C: Merge of panel A and B.
Panel D: Secondary only.
ab235268 has not yet been referenced specifically in any publications.