Key features and details
- Goat polyclonal to BRN3B / POU4F2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-BRN3B / POU4F2 antibody
See all BRN3B / POU4F2 primary antibodies
DescriptionGoat polyclonal to BRN3B / POU4F2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: Rat pup PND2-6 – minimal cortex brain lysate. IHC-P: Mouse testis tissue. ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 43 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionTranscription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
Tissue specificityBrain. Seems to be specific to the retina.
Sequence similaritiesBelongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Cellular localizationNucleus speckle.
- Information by UniProt
- Brain specific homeobox/POU domain protein 3B antibody
- Brain-3B antibody
- Brain-specific homeobox/POU domain protein 3B antibody
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BRN3B / POU4F2 with ab235268 at 1/100 dilution (30 minutes at RT), followed by Donkey anti-Goat (HRP) (4µl/ml, 20 minutes at RT). Primary staining is DAB. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.
Anti-BRN3B / POU4F2 antibody (ab235268) at 1/1000 dilution + Rat pup PND2-6 – minimal cortex brain lysate at 35 µg
Predicted band size: 43 kDa
Exposure time: 12 seconds
Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized Post-natal rat pup (PND1) heterogeneous brain cells labeling BRN3B / POU4F2 with ab235268 at 5 µg/ml (Overnight at 4°C), followed by a Donkey anti-Goat IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 µg/ml (1 hour at RT) (green).
Expected localization: Nuclear, nuclear speckle. The nuclear counterstain is DAPI (blue).
Panel A: DAPI.
Panel B: ab235268 + secondary antibody.
Panel C: Merge of panel A and B.
Panel D: Secondary only.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235268 has not yet been referenced specifically in any publications.