Overview

  • Product name

    Anti-BRN3B / POU4F2 antibody
    See all BRN3B / POU4F2 primary antibodies
  • Description

    Goat polyclonal to BRN3B / POU4F2
  • Host species

    Goat
  • Specificity

    This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459).
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human BRN3B/ POU4F2 aa 327-338 (internal sequence) (Cysteine residue). (NP_004566.2).
    Sequence:

    C-EKSHREKLTKPE


    Database link: Q12837

  • Positive control

    • Flow cyt: MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab240420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 10 µg/ml.

Target

  • Function

    Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
  • Tissue specificity

    Brain. Seems to be specific to the retina.
  • Sequence similarities

    Belongs to the POU transcription factor family. Class-4 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization

    Nucleus speckle.
  • Information by UniProt
  • Database links

  • Alternative names

    • Brain specific homeobox/POU domain protein 3B antibody
    • Brain-3B antibody
    • Brain-specific homeobox/POU domain protein 3B antibody
    • Brn-3B antibody
    • BRN3.2 antibody
    • BRN3B antibody
    • Brn3b POU domain transcription factor antibody
    • class 4 antibody
    • PO4F2_HUMAN antibody
    • POU class 4 homeobox 2 antibody
    • POU domain antibody
    • POU domain class 4 transcription factor 2 antibody
    • POU domain protein antibody
    • POU4F2 antibody
    • transcription factor 2 antibody
    see all

Images

  • Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling BRN3B / POU4F2 with ab240420 at 10 μg/ml for 1 hour (blue) compared with unimmunized goat IgG (black).  An AlexaFluor® 488 anti-Goat IgG was used as the secondary antibody.

References

ab240420 has not yet been referenced specifically in any publications.

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