Product nameAnti-BRN3B / POU4F2 antibody
See all BRN3B / POU4F2 primary antibodies
DescriptionRabbit polyclonal to BRN3B / POU4F2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Rabbit, Horse, Cow, Human, Pig, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
Synthetic peptide corresponding to Mouse BRN3B/ POU4F2 aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: Q63934
- This antibody gave a positive signal in Rat Retina tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab128849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).|
FunctionTranscription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
Tissue specificityBrain. Seems to be specific to the retina.
Sequence similaritiesBelongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Cellular localizationNucleus speckle.
- Information by UniProt
- Brain specific homeobox/POU domain protein 3B antibody
- Brain-3B antibody
- Brain-specific homeobox/POU domain protein 3B antibody
Anti-BRN3B / POU4F2 antibody (ab128849) at 1 µg/ml + Rat Retina Tissue Lysate at 25 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 23 kDa (possible non-specific binding), 53 kDa (possible non-specific binding)
Exposure time: 4 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab128849 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab128849 has not yet been referenced specifically in any publications.