• Product name
    Anti-BRN3B / POU4F2 antibody
    See all BRN3B / POU4F2 primary antibodies
  • Description
    Rabbit polyclonal to BRN3B / POU4F2
  • Host species
  • Specificity
    This antibody reacts with BRN3B / POU4F2.
  • Tested applications
    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human BRN3B/ POU4F2 aa 1-50 (N terminal).


  • Positive control
    • WB: HepG2 cell lysate. IF/ICC: SKNSH cell line



Our Abpromise guarantee covers the use of ab56026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

WB Use a concentration of 0.2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
  • Tissue specificity
    Brain. Seems to be specific to the retina.
  • Sequence similarities
    Belongs to the POU transcription factor family. Class-4 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization
    Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain specific homeobox/POU domain protein 3B antibody
    • Brain-3B antibody
    • Brain-specific homeobox/POU domain protein 3B antibody
    • Brn-3B antibody
    • BRN3.2 antibody
    • BRN3B antibody
    • Brn3b POU domain transcription factor antibody
    • class 4 antibody
    • PO4F2_HUMAN antibody
    • POU class 4 homeobox 2 antibody
    • POU domain antibody
    • POU domain class 4 transcription factor 2 antibody
    • POU domain protein antibody
    • POU4F2 antibody
    • transcription factor 2 antibody
    see all


  • ICC/IF image of ab56026 stained Malme-3M cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56026, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-BRN3B / POU4F2 antibody (ab56026) at 0.2 µg/ml + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 43 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)


This product has been referenced in:
  • Liu Y  et al. Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells. Int J Mol Med 40:1172-1184 (2017). Read more (PubMed: 28848998) »
  • Sluch VM  et al. Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line. Sci Rep 5:16595 (2015). Read more (PubMed: 26563826) »

See all 3 Publications for this product

Customer reviews and Q&As

Please forward this email to your customer:

Thank you very much for your interest our products.

To our knowledge, we do not have an alternative antibody which has been tested in IHC so far, although we have two mor...

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Thank you for your telephone enquiry yesterday afternoon.

I am sorry to confirm that ab32264 BRN3B / POU4F2 antibodyis not longer available.

As discussed on the telephone, we do have an alternative, ab56026. I can recommend to rev...

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