• Product name
  • Description
    Rabbit polyclonal to BRN4
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Horse, Cow, Human, Pig, Macaque monkey, Chinese hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Rat BRN4.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysayes: Rat Brain; Rat Hippocampus; Rat Cerebellum.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab104562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IHC-P Use a concentration of 5 µg/ml.


  • Function
    Probable transcription factor which exert its primary action widely during early neural development and in a very limited set of neurons in the mature brain.
  • Tissue specificity
    Brain specific.
  • Involvement in disease
    Defects in POU3F4 are a cause of deafness X-linked type 2 (DFNX2) [MIM:304400]. A mixed type of deafness characterized by both conductive hearing loss resulting from stapes (perilymphatic gusher) fixation and progressive sensorineural deafness.
  • Sequence similarities
    Belongs to the POU transcription factor family. Class-3 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain specific homeobox POU domain protein 4 antibody
    • Brain-4 antibody
    • Brain-specific homeobox/POU domain protein 4 antibody
    • BRAIN4 antibody
    • Brn-4 antibody
    • BRN4 antibody
    • class 3 antibody
    • DFN3 antibody
    • DFNX2 antibody
    • Oct-9 antibody
    • Octamer-binding protein 9 antibody
    • Octamer-binding transcription factor 9 antibody
    • OTF-9 antibody
    • OTF9 antibody
    • PO3F4_HUMAN antibody
    • POU class 3 homeobox 4 antibody
    • POU domain antibody
    • POU domain class 3 transcription factor 4 antibody
    • Pou3f4 antibody
    • transcription factor 4 antibody
    see all


  • All lanes : Anti-BRN4 antibody (ab104562) at 1 µg/ml

    Lane 1 : Rat Hippocampus Tissue Lysate
    Lane 2 : Cerebellum Rat Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 39 kDa
    Additional bands at: 190 kDa, 95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 8 minutes
  • IHC image of BRN4 staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104562, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


ab104562 has not yet been referenced specifically in any publications.

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