Product nameBromocresol purple, pH indicator
DescriptionpH indicator; vital stain.
- Bromcresol purple sultone form
Triphenylmethane dye used as a pH indicator, colored yellow below pH 5.2, and violet above pH 6.8. Also used in the spectrophotometric determination of serum-albumin, and as a fluorescent stain in yeast for dead cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Lindsay AK et al. Analysis of Candida albicans mutants defective in the Cdk8 module of mediator reveal links between metabolism and biofilm formation. PLoS Genet 10:e1004567 (2014). Read more (PubMed: 25275466) »
- Iskander I et al. Serum bilirubin and bilirubin/albumin ratio as predictors of bilirubin encephalopathy. Pediatrics 134:e1330-9 (2014). Read more (PubMed: 25332491) »
- Kurzweilová H & Sigler K Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity. Yeast 9:1207-11 (1993). Read more (PubMed: 7509098) »
- Affonso A & Lasky FD Bromocresol purple dye-binding method for the estimation of serum albumin adapted to the SMA 12/60. Clin Biochem 18:285-9 (1985). Read more (PubMed: 4053341) »
- Hill PG & Wells TN Bromocresol purple and the measurement of albumin. Falsely high plasma albumin concentrations eliminated by increased reagent ionic strength. Ann Clin Biochem 20 (Pt 5):264-70 (1983). Read more (PubMed: 6651190) »