Key features and details
- Rabbit polyclonal to BROX
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BROX antibody
DescriptionRabbit polyclonal to BROX
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- ICC/IF: U-2 OS cells. IHC-P: Human urinary bladder tissue. WB: RT4 and U-251 MG cell lysate. Human liver and tonsil tissue lysate.
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Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Sequence similaritiesBelongs to the BROX family.
Contains 1 BRO1 domain.
- Information by UniProt
- BRO1 domain and CAAX motif containing antibody
- BRO1 domain containing protein antibody
- BRO1 domain- and CAAX motif-containing protein antibody
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab122174 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122174, at 1/500 dilution, staining BROX in paraffin-embedded human urinary bladder tissue by Immunohistochemistry.
All lanes : Anti-BROX antibody (ab122174) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab122174 has not yet been referenced specifically in any publications.