Overview

  • Product name

    Anti-BRRN1 antibody
  • Description

    Rabbit polyclonal to BRRN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 439-741 of Human BRRN1 (Uniprot ID: Q15003).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; Human gastric carcinoma tissue; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab154105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • Tissue specificity

    Widely expressed at low level. Expressed in proliferating cells.
  • Sequence similarities

    Belongs to the CND2 (condensin subunit 2) family.
  • Post-translational
    modifications

    Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPG subunits, activates the condensin complex and is required for chromosome condensation.
  • Cellular localization

    Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • Barren Drosophila Homolog of 1 antibody
    • Barren homolog protein 1 antibody
    • BRRN antibody
    • BRRN1 antibody
    • CAP H antibody
    • CAPH antibody
    • Chromosome associated protein H antibody
    • Chromosome-associated protein H antibody
    • CND2_HUMAN antibody
    • Condensin complex subunit 2 antibody
    • Condensin subunit 2 antibody
    • Condensin-2 complex subunit H2 antibody
    • Condesin antibody
    • HCAP H antibody
    • hCAP-H antibody
    • KIAA0074 antibody
    • mCAP H antibody
    • MGC4616 antibody
    • Ncaph antibody
    • Non SMC condensin I complex subunit H antibody
    • Non-SMC condensin I complex subunit H antibody
    • XCAP H homolog antibody
    • XCAP-H homolog antibody
    see all

Images

  • Anti-BRRN1 antibody (ab154105) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 83 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling BRRN1 with ab154105 at 1/200 dilution. Lower image show cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling BRRN1 with ab154105 at 1/500 dilution.

References

ab154105 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (fibroblast)
Loading amount
50 µg
Specification
fibroblast
Gel Running Conditions
Reduced Denaturing (7%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 01 2013

Answer

I am very pleased to hear you would like to test ab31865, ab122555, ab136973, and ab154105 in mouse. These codes will give you the value listed above off future orders before the expiration date. To redeem this offer, please submit an Abreview for mouse for each antibody and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

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