Anti-BRRN1 antibody (ab154105)
Key features and details
- Rabbit polyclonal to BRRN1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BRRN1 antibody -
Description
Rabbit polyclonal to BRRN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 439-741 of Human BRRN1 (Uniprot ID: Q15003).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; Human gastric carcinoma tissue; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 83 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 83 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. -
Tissue specificity
Widely expressed at low level. Expressed in proliferating cells. -
Sequence similarities
Belongs to the CND2 (condensin subunit 2) family. -
Post-translational
modificationsPhosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPG subunits, activates the condensin complex and is required for chromosome condensation. -
Cellular localization
Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. - Information by UniProt
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Database links
- Entrez Gene: 23397 Human
- Omim: 602332 Human
- SwissProt: Q15003 Human
- Unigene: 308045 Human
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Alternative names
- Barren Drosophila Homolog of 1 antibody
- Barren homolog protein 1 antibody
- BRRN antibody
see all
Images
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Anti-BRRN1 antibody (ab154105) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 83 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling BRRN1 with ab154105 at 1/200 dilution. Lower image show cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRRN1 antibody (ab154105)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling BRRN1 with ab154105 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154105 has been referenced in 2 publications.
- Fitz-James MH et al. Large domains of heterochromatin direct the formation of short mitotic chromosome loops. Elife 9:N/A (2020). PubMed: 32915140
- Lorenzon I et al. Identification and Characterization of a New Platinum-Induced TP53 Mutation in MDAH Ovarian Cancer Cells. Cells 9:N/A (2019). PubMed: 31877751