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Anti-BRRN1 antibody (ab154105)

Reviews (1)Q&A (1)References (2)
Western blot - Anti-BRRN1 antibody (ab154105)
  • Immunocytochemistry/ Immunofluorescence - Anti-BRRN1 antibody (ab154105)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRRN1 antibody (ab154105)

Key features and details

  • Rabbit polyclonal to BRRN1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-BRRN1 antibody

  • Description

    Rabbit polyclonal to BRRN1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 439-741 of Human BRRN1 (Uniprot ID: Q15003).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; Human gastric carcinoma tissue; HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 83 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 83 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

  • Tissue specificity

    Widely expressed at low level. Expressed in proliferating cells.

  • Sequence similarities

    Belongs to the CND2 (condensin subunit 2) family.

  • Post-translational
    modifications

    Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPG subunits, activates the condensin complex and is required for chromosome condensation.

  • Cellular localization

    Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt

  • Database links

    • Alternative names

      • Barren Drosophila Homolog of 1 antibody
      • Barren homolog protein 1 antibody
      • BRRN antibody
      • BRRN1 antibody
      • CAP H antibody
      • CAPH antibody
      • Chromosome associated protein H antibody
      • Chromosome-associated protein H antibody
      • CND2_HUMAN antibody
      • Condensin complex subunit 2 antibody
      • Condensin subunit 2 antibody
      • Condensin-2 complex subunit H2 antibody
      • Condesin antibody
      • HCAP H antibody
      • hCAP-H antibody
      • KIAA0074 antibody
      • mCAP H antibody
      • MGC4616 antibody
      • Ncaph antibody
      • Non SMC condensin I complex subunit H antibody
      • Non-SMC condensin I complex subunit H antibody
      • XCAP H homolog antibody
      • XCAP-H homolog antibody
      see all

    Images

    • Western blot - Anti-BRRN1 antibody (ab154105)
      Western blot - Anti-BRRN1 antibody (ab154105)
      Anti-BRRN1 antibody (ab154105) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

      Predicted band size: 83 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-BRRN1 antibody (ab154105)
      Immunocytochemistry/ Immunofluorescence - Anti-BRRN1 antibody (ab154105)
      Immunofluorescent analysis of methanol-fixed HeLa cells labeling BRRN1 with ab154105 at 1/200 dilution. Lower image show cells co-stained with Hoechst 33342.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRRN1 antibody (ab154105)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRRN1 antibody (ab154105)
      Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling BRRN1 with ab154105 at 1/500 dilution.

    References (2)

    Publishing research using ab154105? Please let us know so that we can cite the reference in this datasheet.

    ab154105 has been referenced in 2 publications.

    • Fitz-James MH  et al. Large domains of heterochromatin direct the formation of short mitotic chromosome loops. Elife 9:N/A (2020). PubMed: 32915140
    • Lorenzon I  et al. Identification and Characterization of a New Platinum-Induced TP53 Mutation in MDAH Ovarian Cancer Cells. Cells 9:N/A (2019). PubMed: 31877751

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-BRRN1 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (fibroblast)
    Loading amount
    50 µg
    Specification
    fibroblast
    Gel Running Conditions
    Reduced Denaturing (7%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 01 2013

    Question

    I will also be detecting murine CAP-H (BRRN1). Is it possible to be enrolled in AbTrial program if I order ab136973 and/or ab154105?

    Read More
    Answer

    I am very pleased to hear you would like to test ab31865, ab122555, ab136973, and ab154105 in mouse. These codes will give you the value listed above off future orders before the expiration date. To redeem this offer, please submit an Abreview for mouse for each antibody and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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