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    brs-3-antibody-ab75149.pdf

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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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Anti-BRS-3 antibody (ab75149)

  • Datasheet
Reviews (2)Q&A (1)

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Abpromise

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Western blot - Anti-BRS-3 antibody (ab75149)

    Key features and details

    • Rabbit polyclonal to BRS-3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-BRS-3 antibody
      See all BRS-3 primary antibodies
    • Description

      Rabbit polyclonal to BRS-3
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human BRS-3 (internal sequence).

    • Positive control

      • Extracts from HUVEC cells.
    • General notes

      Previously labelled as Bombesin Receptor 3.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS

      Without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • GPCR
      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab75149 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB (2)
    1/500 - 1/1000. Predicted molecular weight: 44 kDa.
    Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 44 kDa.

    Target

    • Function

      Role in sperm cell division, maturation, or function. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
    • Tissue specificity

      In germ cells in testis. Lung carcinoma cells.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P32247 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 680 Human
      • Omim: 300107 Human
      • SwissProt: P32247 Human
      • Unigene: 121484 Human
      • Alternative names

        • BB3 antibody
        • BLP receptor subtype 3 antibody
        • Bombesin like receptor 3 antibody
        • Bombesin Receptor 3 antibody
        • Bombesin receptor subtype 3 antibody
        • Bombesin receptor subtype-3 antibody
        • BRS 3 antibody
        • BRS-3 antibody
        • BRS3 antibody
        • BRS3_HUMAN antibody
        see all

      Images

      • Western blot - Anti-BRS-3 antibody (ab75149)
        Western blot - Anti-BRS-3 antibody (ab75149)
        All lanes : Anti-BRS-3 antibody (ab75149) at 1/500 dilution

        Lane 1 : Extracts from HUVEC cells
        Lane 2 : Extracts from HUVEC cells with immunising peptide at 5 µg

        Lysates/proteins at 15 µg per lane.

        Predicted band size: 44 kDa
        Observed band size: 47 kDa
        why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab75149? Please let us know so that we can cite the reference in this datasheet.

      ab75149 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Question

      Customer has kindly contacted us to inquire whether this product targets extracellular or cytoplasmid domain of BRS3.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 03 2012

      Answer

      Thank you for contacting us and your interest in our products.


      I am pleased to provide the following immunogen information:

      The amino acid range of this immunogen is aa180-230 of human Bombesin receptor subtype-3 with accession number of P32247(http://www.uniprot.org/uniprot/P32247). This antibody binds to the extracellular topological domain (based on prediction).


      I hope this information has been of help to you. If you have any further questions, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on Oct 03 2012

      Western blot abreview for Anti-Bombesin Receptor 3 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (STC1)
      Loading amount
      20 µg
      Specification
      STC1
      Gel Running Conditions
      Reduced Denaturing (10% gel)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Dr. Rob Tolhurst

      Verified customer

      Submitted Sep 20 2010

      Western blot abreview for Anti-Bombesin Receptor 3 antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEK293, HGT101, CaCo2)
      Loading amount
      200000 cells
      Specification
      HEK293, HGT101, CaCo2
      Gel Running Conditions
      Reduced Denaturing (10% gel)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
      Read More

      Dr. Rob Tolhurst

      Verified customer

      Submitted Aug 20 2010

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