Recombinant Anti-BRSK1 antibody [EPR18190] (ab206298)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18190] to BRSK1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-BRSK1 antibody [EPR18190]
See all BRSK1 primary antibodies -
Description
Rabbit monoclonal [EPR18190] to BRSK1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y, Jurkat, PC-12 and NIH/3T3 whole cell lysates; mouse testis lysate; rat brain lysate. ICC/IF: Neuro-2a and NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18190 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
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WB |
1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
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Notes |
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ICC/IF
1/100. |
WB
1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
Target
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Function
Required for the polarization of forebrain neurons which endows axons and dendrites with distinct properties, possibly by locally regulating phosphorylation of microtubule-associated proteins (By similarity). May be involved in the regulation of G2/M arrest in response to UV- or methyl methane sulfonate (MMS)-induced, but not IR-induced, DNA damage. Phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo. -
Tissue specificity
Widely expressed, with highest levels in brain and testis. Protein levels remain constant throughout the cell cycle. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
Cellular localization
Cytoplasm. Nucleus. Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 84446 Human
- Entrez Gene: 381979 Mouse
- Entrez Gene: 499073 Rat
- Omim: 609235 Human
- SwissProt: Q8TDC3 Human
- SwissProt: Q5RJI5 Mouse
- SwissProt: B2DD29 Rat
- Unigene: 182081 Human
see all -
Alternative names
- BR serine/threonine kinase 1 antibody
- BR serine/threonine protein kinase 1 antibody
- BR serine/threonine-protein kinase 1 antibody
see all
Images
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All lanes : Anti-BRSK1 antibody [EPR18190] (ab206298) at 1/1000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : Mouse testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-BRSK1 antibody [EPR18190] (ab206298) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-BRSK1 antibody [EPR18190] (ab206298) at 1/1000 dilution
Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDaExposure time: Lane 1 - 30 seconds; Lane 2 - 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling BRSK1 with ab206298 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing both nuclear and cytoplasmic staining on Neuro-2a cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows;
-ve control 1: ab206298 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution -
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling BRSK1 with ab206298 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing both nuclear and cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows;
-ve control 1: ab206298 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab206298 has not yet been referenced specifically in any publications.