Product nameAnti-BRUNOL4 antibody [EPR11824]
DescriptionRabbit monoclonal [EPR11824] to BRUNOL4
Tested applicationsSuitable for: WB, IHC-P, IP, Flow Cytmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human BRUNOL4. The exact sequence is proprietary.
Database link: Q9BZC1
- WI-38, A431, HeLa, NIH:OVCAR-3 and SH-SY5Y cell lysates; Human pancreas and kidney tissues; permeabilized HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 52 kDa.|
|IHC-P||1/50 - 1/100.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceBRUNOL4 is an RNA binding protein implicated in the regulation of pre mRNA alternative splicing. It mediates exon inclusion and/or exclusion in pre mRNA that are subject to tissue specific and developmentally regulated alternative splicing. It specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition; promotes exclusion of both the smooth muscle (SM) and non muscle (NM) exons in actinin pre mRNAs; binds to muscle specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre mRNA.
Cellular localizationCytoplasmic and Nuclear
- bruno like 4, RNA binding protein antibody
- Bruno like 4, RNA binding protein (Drosophila) antibody
- Bruno like protein 4 antibody
Flow cytometric analysis of permeabilized Hela cells labeling BRUNOL4 using ab171740 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunohistochemistry of paraffin-embedded Human kidney tissue labeling BRUNOL4 using ab171740 at 1/50 dilution.
Immunohistochemistry of paraffin-embedded Human pancreas tissue labeling BRUNOL4 using ab171740 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate labeling BRUNOL4 or (2) 1X PBS (negative control) using ab171740 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes : Anti-BRUNOL4 antibody [EPR11824] (ab171740) at 1/1000 dilution
Lane 1 : WI-38 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NIH:OVCAR-3 cell lysate
Lane 5 : SH-SY5Y cell lysate
Lysates/proteins at 1/10 dilution per lane.
Predicted band size: 52 kDa
ab171740 has not yet been referenced specifically in any publications.