Product nameAnti-BS69 antibody
See all BS69 primary antibodies
DescriptionRabbit polyclonal to BS69
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rhesus monkey
Recombinant fragment within Human BS69 (internal sequence). The exact sequence is proprietary.
Database link: Q15326
- WB: BS69 transfected HEK-293T cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab227086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 71 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
RelevanceBS69 is a factor previously identified as an adenovirus E1A-associated protein. The protein localizes to the nucleus. It functions as a transcriptional repressor, and expression of E1A inhibits this repression. Two transcript variants encoding different isoforms have been found for this gene.
- Adenovirus 5 E1A binding protein antibody
- Bone morphogenetic protein receptor associated molecule 1 antibody
- BRAM1 antibody
All lanes : Anti-BS69 antibody (ab227086) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : BS69 transfected HEK-293T cell lysate
Predicted band size: 71 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded human colon carcinoma tissue stained for BS69 using ab227086 at 1/250 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for BS69 (green) using ab227086 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
ab227086 has not yet been referenced specifically in any publications.