Key features and details
- Rabbit polyclonal to BS69
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BS69 antibody
See all BS69 primary antibodies
DescriptionRabbit polyclonal to BS69
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rhesus monkey
Recombinant fragment within Human BS69 (internal sequence). The exact sequence is proprietary.
Database link: Q15326
- WB: BS69 transfected HEK-293T cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab227086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 71 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
RelevanceBS69 is a factor previously identified as an adenovirus E1A-associated protein. The protein localizes to the nucleus. It functions as a transcriptional repressor, and expression of E1A inhibits this repression. Two transcript variants encoding different isoforms have been found for this gene.
- Adenovirus 5 E1A binding protein antibody
- Bone morphogenetic protein receptor associated molecule 1 antibody
- BRAM1 antibody
All lanes : Anti-BS69 antibody (ab227086) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : BS69 transfected HEK-293T cell lysate
Predicted band size: 71 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded human colon carcinoma tissue stained for BS69 using ab227086 at 1/250 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for BS69 (green) using ab227086 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227086 has not yet been referenced specifically in any publications.