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    bsa-removal-kit-ab173231.pdf

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Kits/ Lysates/ Other Kits Molecular Biology Kits Purification Kit BSA removal
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BSA Removal Kit (ab173231)

  • Datasheet
  • Protocol Booklet
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ab173231 BSA Removal Kit

    Key features and details

    • Assay type: Direct
    • Assay time: 10 min

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    Overview

    • Product name

      BSA Removal Kit
    • Assay type

      Direct
    • Assay time

      0h 10m
    • Product overview

      Abcam’s BSA Removal Kit (ab173231) is a simple one-step, 10 minute method which effectively separates the BSA from the antibody. The antibody is left in a suitable position for transfer to a buffer more suited to conjugation. The BSA Removal Kit can be used on any antibody sub-type, and species.


      This 1 ml kit can remove all of the BSA from up to 1.25 ml of antibody, with a BSA concentration of 0.5% or less. For higher BSA  concentrations, the method may need to be repeated, or a higher volume of BSA Removal Buffer may be required.


      Please note that this kit is not compatible with our Gold, Latex, Europium and Magnetic Conjugation Kits. To remove BSA from antibodies prior to conjugation with these kits, please use BSA Removal Kit - Nanoparticles (ab204912).


      Important considerations:



      • The BSA Removal Kit can separate BSA from antibody solutions with antibody concentrations from 0.03 mg/mL to 10 mg/mL Separation is more efficient at higher antibody concentrations. 50 µg of antibody is the lower limit for seeing a clearly visible pellet.

      • BSA can be effectively separated when present at concentrations of up to 0.5%. If BSA is present at higher concentrations, dilute the antibody mix with de-ionized, distilled water until BSA concentration is 0.5% or less. Alternatively, if BSA is over a 0.5%, two or more runs may need to be performed to completely remove BSA. 

      • Glycerol concentration must not exceed 20%.

      • The components of the removal buffer may precipitate at low temperatures. Gently warming the solution should allow solubilisation. Any undissolved crystals should be spun down by brief centrifugation, and the supernatant should be used for the BSA removal.

    • Notes

      This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect BSA Removal Kit. 820-0100 is the same as the 1 ml size.

      Bovine Serum Albumin (BSA) is often added to purified antibodies as it is an effective stabilizer. However, when labelling antibodies, the BSA becomes a hindrance, as it directly competes with the antibody to attach to the label, greatly reducing the conjugation efficiency. Therefore, prior to undertaking labelling techniques, it is essential to remove the BSA. Common commercial BSA removal techniques can involve many laborious steps.

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 ml
      ab274171 - BSA Removal Buffer 1 x 1ml
      ab274172 - Re-suspension Buffer 1 x 500µl
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Molecular Biology Kits
      • Purification Kit
      • BSA removal

    Images

    • ab173231 BSA Removal Kit
      ab173231 BSA Removal Kit

      SDS-PAGE Gel showing the use of ab173231 BSA Removal Kit on a mixture containing 1 mg/ml IgG and 1 mg/ml BSA. The gel shows the mix before and after separation. 4-12% Bis-Tris gel, non-reducing.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab173231? Please let us know so that we can cite the reference in this datasheet.

    ab173231 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    BSA removal from antibody

    Excellent Good 4/5 (Ease of Use)
    Abreviews
    Abreviews
    This kit was used to remove BSA from antibody in order to label the antibody with AlexaFluor 647 dye. The labeled antibody was successfully used in a functional assay, so the BSA removal was successful.

    I found that the BSA removal buffer had a significant amount of precipitate in it and it took a lot of heating and vortexing to solubilize it. The product sheet gives instructions on how to solubilize it. However, it recommends heating to 40°C and says to not heat to over 44°C. Unless you have a very precise heat block or something similar to ensure the temperature is not over 44°C, I would recommend heating at 37°C. Other than the resuspension of the BSA removal buffer, the kit was very easy to use.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 21 2022

    TRAS BON PRODUIT

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    PERFECT ANTIBODY
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 24 2018

    Question


    Inquiry: WE have a FITC-conjugated primary antibody and would like to use your BSA removal kit. I was wondering if there would be any issues with the FITC and the kit itself?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 09 2016

    Answer

    The ab173231 BSA Removal Kit purification system is based on the molecule size. We have never tested the kit for an already-conjugated antibody.

    Was the antibody conjugated to FITC using our Abcam FITC conjugation kit? If so, the way it processes the molecules of FITC might affect the solubility of the protein in this salt-induced precipitation. We have never tried putting any pre-conjugated protein through the kit so we cannot really guarantee a successful purification with the BSA removal kit.

    If the antibody was already conjugated to FITC using a different kit working with different technology, I cannot say for sure if it will work. The only main consideration here is how the molecule with interfere with salt-based precipitation.

    I’m sorry I can’t be of much more help, If you do decide to try the kit I will be interested to hear from you with an update on how successful it was.

    Read More

    Abcam Scientific Support

    Answered on Nov 09 2016

    Question


    Inquiry: Can this product be used to remove BSA from cell culture medium?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 07 2015

    Answer



    The BSA removal kit would not be appropriate for in this case because cell culture medium will contain many other proteins which will not be removed by this kit

    To purify the antibody from tissue culture supernatant I can suggest to consider one of the purification kits.

    I am sorry I do not have details of the particular antibody you are purifying, but the following sound be suitable for purification from tissue culture supernatant. I can recommend to review the details to see if it would be suitable and let me know kf you have any questions.

    Antibody TCS Purification Kit (ab109207)
    https://www.abcam.com/TCS-Antibody-Purification-Kit-ab109207.html (or use the following: https://www.abcam.com/TCS-Antibody-Purification-Kit-ab109207.html).

    Read More

    Sam Washer

    Abcam Scientific Support

    Answered on Dec 07 2015

    Question

    Can this kit be used on rat/monkey serum to remove albumin?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 14 2014

    Answer

    The lab have confirmed that the BSA removal kit ab173231 will be suitable for removing Albumin from both the rat and monkey serum samples. It is however worth noting that, due to the nature of the purification, it is probable that some of the other buffer components will also be removed.

    Read More

    Elisa Thomas

    Abcam Scientific Support

    Answered on Mar 14 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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