BSA Removal Kit (ab173231)
- Datasheet
- References
- Protocols
Overview
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Product name
BSA Removal Kit -
Assay type
Direct -
Assay time
0h 10m -
Product overview
This product is manufactured by Expedeon, an Abcam company. Expedeon product code 820-0100 was previously called AbSelect BSA Removal Kit 1 ml and is the same as the 1 ml size of this product.
Abcam’s BSA Removal Kit (ab173231) is a simple one-step, 10 minute method which effectively separates the BSA from the antibody. The antibody is left in a suitable position for transfer to a buffer more suited to conjugation. The BSA Removal Kit can be used on any antibody sub-type, and species.
This 1 ml kit can remove all of the BSA from up to 1.25 ml of antibody, with a BSA concentration of 0.5% or less. For higher BSA concentrations, the method may need to be repeated, or a higher volume of BSA Removal Buffer may be required.
Please note that this kit is not compatible with our range of GOLD conjugation kits. To remove BSA from antibodies prior to conjugation with our GOLD conjugation kits please use GOLD BSA removal kit (ab204912).
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Notes
Bovine Serum Albumin (BSA) is often added to purified antibodies as it is an effective stabilizer. However, when labelling antibodies, the BSA becomes a hindrance, as it directly competes with the antibody to attach to the label, greatly reducing the conjugation efficiency. Therefore, prior to undertaking labelling techniques, it is essential to remove the BSA. Common commercial BSA removal techniques can involve many laborious steps.
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Tested applications
Suitable for: Conjugationmore details
Properties
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Storage instructions
Store at room temperature. Please refer to protocols. -
Components 1 ml BSA Removal Buffer 1 vial Re-suspension Buffer 1 vial -
Research areas
Applications
Our Abpromise guarantee covers the use of ab173231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Conjugation | Use at an assay dependent concentration. Removes all of the BSA from up to 1.25 ml of antibody, with a BSA concentration of 0.5% or less. |
Images
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ab173231 BSA Removal KitSDS-PAGE Gel showing the use of ab173231 BSA Removal Kit on a mixture containing 1 mg/ml IgG and 1 mg/ml BSA. The gel shows the mix before and after separation. 4-12% Bis-Tris gel, non-reducing.
Protocols
References
ab173231 has not yet been referenced specifically in any publications.
