BSA Removal Kit (ab173231)
- Datasheet
- References
- Protocols
Overview
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Product nameBSA Removal Kit
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Assay typeDirect
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Assay time0h 10m
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Product overview
Abcam’s BSA Removal Kit (ab173231) is a simple one-step, 10 minute method which effectively separates the BSA from the antibody. The antibody is left in a suitable position for transfer to a buffer more suited to conjugation. The BSA Removal Kit can be used on any antibody sub-type, and species.
This 1 ml kit can remove all of the BSA from up to 1.25 ml of antibody, with a BSA concentration of 0.5% or less. For higher BSA concentrations, the method may need to be repeated, or a higher volume of BSA Removal Buffer may be required.
Please note that this kit is not compatible with our range of GOLD conjugation kits. To remove BSA from antibodies prior to conjugation with our GOLD conjugation kits please use GOLD BSA removal kit (ab204912).
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Notes
Bovine Serum Albumin (BSA) is often added to purified antibodies as it is an effective stabilizer. However, when labelling antibodies, the BSA becomes a hindrance, as it directly competes with the antibody to attach to the label, greatly reducing the conjugation efficiency. Therefore, prior to undertaking labelling techniques, it is essential to remove the BSA. Common commercial BSA removal techniques can involve many laborious steps.
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Tested applicationsSuitable for: Conjugationmore details
Properties
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Storage instructionsStore at room temperature. Please refer to protocols.
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Components 1 ml BSA Removal Buffer 1 vial Re-suspension Buffer 1 vial -
Research areas
Applications
Our Abpromise guarantee covers the use of ab173231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Conjugation | Use at an assay dependent concentration. Removes all of the BSA from up to 1.25 ml of antibody, with a BSA concentration of 0.5% or less. |
Images
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ab173231 BSA Removal KitSDS-PAGE Gel showing the use of ab173231 BSA Removal Kit on a mixture containing 1 mg/ml IgG and 1 mg/ml BSA. The gel shows the mix before and after separation
Protocols
References
ab173231 has not yet been referenced specifically in any publications.
