- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-BSD antibody
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DescriptionRabbit polyclonal to BSD
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Host speciesRabbit
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SpecificityThe antibody detects a 13 kDa protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells.
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Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
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Species reactivitynot applicable
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Immunogen
Synthetic peptide conjugated to KLH corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase from Aspergillus Terrus.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThis antibody was affinity purified using the N-terminal Bsd peptide (without carrier).
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab38307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Detects a band of approximately 13 kDa. | |
| ELISA | 1/4000. | |
| ICC/IF | 1/100. |
Target
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RelevanceRetroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week.
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Alternative names
- Blasticidin-S deaminase antibody
- BSD antibody
Images
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Western blot - Anti-BSD antibody (ab38307)All lanes : Anti-BSD antibody (ab38307)
Lane 1 : 293 cells mock transfected
Lanes 2-3 : 293 cells transiently transfected with pLenti6/TR lentiviral vector
Observed band size: 13 kDa (why is the actual band size different from the predicted?)
Each lane was loaded with 10-20µg total protein. -
Immunocytochemistry/ Immunofluorescence - Anti-BSD antibody (ab38307)Image courtesy of Charles Mashburn and Dr. George Smith at the University of Kentucky, Spinal Cord and Brain Injury Research Centerab38307, staining BSD in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were fixed in paraformaldehyde (3.75%) for 30 min., then permeabilized in 0.2% Triton X-100 for 15 min. Blocking was done using 1% BSA for 30 min. The primary was used at 1/100 and then an appropriate secondary antibody conjugated to Texas Red was used.
References
This product has been referenced in:
- Jammart B et al. Very-low-density lipoprotein (VLDL)-producing and hepatitis C virus-replicating HepG2 cells secrete no more lipoviroparticles than VLDL-deficient Huh7.5 cells. J Virol 87:5065-80 (2013). Read more (PubMed: 23427158) »
- de Wispelaere M & Yang PL Mutagenesis of the DI/DIII linker in dengue virus envelope protein impairs viral particle assembly. J Virol 86:7072-83 (2012). Read more (PubMed: 22532681) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"