Recombinant
RabMAb

Recombinant Anti-BSND antibody [EPR14270] - C-terminal (ab196017)

Overview

  • Product name
    Anti-BSND antibody [EPR14270] - C-terminal
    See all BSND primary antibodies
  • Description
    Rabbit monoclonal [EPR14270] to BSND - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BSND aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8WZ55

  • Positive control
    • Human fetal kidney, Mouse spleen and Rat kidney lysates; Human kidney, Rat kidney and Mouse kidney tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14270
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    Functions as a beta-subunit for CLCNKA and CLCNKB chloride channels. In the kidney CLCNK/BSND heteromers mediate chloride reabsorption by facilitating its basolateral efflux. In the stria, CLCNK/BSND channels drive potassium secretion by recycling chloride for the basolateral SLC12A2 cotransporter.
  • Tissue specificity
    Expressed primarily in kidney. Expressed in specific nephron segments and in the stria vascularis of the inner ear.
  • Involvement in disease
    Bartter syndrome 4A
  • Cellular localization
    Cell membrane. Cytoplasm. A significant amount also observed intracellularly. Staining in membranes of the renal tubule and of potassium-secreting epithelia of the inner ear is basolateral.
  • Information by UniProt
  • Database links
  • Alternative names
    • BART antibody
    • Bartter syndrome infantile with sensorineural deafness antibody
    • Bartter syndrome, infantile, with sensorineural deafness (Barttin) antibody
    • Barttin antibody
    • Bsnd antibody
    • BSND_HUMAN antibody
    • deafness, autosomal recessive 73 antibody
    • DFNB 73 antibody
    • DFNB73 antibody
    see all

Images

  • All lanes : Anti-BSND antibody [EPR14270] - C-terminal (ab196017) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.



    This protein expressed primarily in kidney.

    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling BSND with ab196017 at 1/2000. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

    Inset image: negative control obtained using PBS instead of ab196017 and secondary antibody only.
    Note: Membrane and cytoplasm staining on human kidney tissue was observed.

  • Anti-BSND antibody [EPR14270] - C-terminal (ab196017) at 1/1000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.



    This protein is expressed primarily in kidney.

    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling BSND with ab196017 at 1/2000. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

    Inset image: negative control obtained using PBS instead of ab196017 and secondary antibody only.
    Note: Membrane and cytoplasm staining on mouse kidney tissue was observed.

  • Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling BSND with ab196017 at 1/2000. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

    Inset image: negative control obtained using PBS instead of ab196017 and secondary antibody only.
    Note: Membrane and cytoplasm staining on rat kidney tissue was observed.

References

ab196017 has not yet been referenced specifically in any publications.

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