Overview

  • Product name

    Anti-BST2/Tetherin antibody
    See all BST2/Tetherin primary antibodies
  • Description

    Mouse polyclonal to BST2/Tetherin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human BST2/Tetherin.
    Database link: NP_004326.1

  • Positive control

    • Human spleen tissue lysate, Jurkat cell lysate and BST2/Tetherin transfected 293T cell lysate Human kidney tissue HeLa cell
  • General notes

     This product was previously labelled as BST2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab88523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis.
  • Tissue specificity

    Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level).
  • Sequence similarities

    Belongs to the tetherin family.
  • Domain

    The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading.
  • Post-translational
    modifications

    Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation.
  • Cellular localization

    Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bone marrow stromal antigen 2 antibody
    • Bone marrow stromal cell antigen 2 antibody
    • Bone marrow stromal cell antigen antibody
    • BST 2 antibody
    • BST-2 antibody
    • BST2 antibody
    • BST2_HUMAN antibody
    • CD 317 antibody
    • CD317 antibody
    • CD317 antigen antibody
    • HM1.24 antigen antibody
    • NPC A 7 antibody
    • Tetherin antibody
    see all

Images

  • Anti-BST2/Tetherin antibody (ab88523) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size: 20 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?

  • Anti-BST2/Tetherin antibody (ab88523) at 1 µg/ml + jurkat cell lysate at 50 µg

    Predicted band size: 20 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?

  • ab88523, at 3 µg/ml, staining BST2/Tetherin in formalin fixed, paraffin embedded human kidney tissue sections by Immunoperoxidase/Immunohistochemistry.

  • ab88523 at 10 µg/ml staining BST2/Tetherin in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.

References

This product has been referenced in:

  • Zou J  et al. Correction: The viral restriction factor Tetherin prevents leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) from association with Beclin 1 and B-cell CLL/lymphoma 2 (Bcl-2) and enhances autophagy and mitophagy. J Biol Chem 294:5211 (2019). Read more (PubMed: 30926759) »
  • Teng Y  et al. CRISPR/Cas9-mediated deletion of miR-146a enhances antiviral response in HIV-1 infected cells. Genes Immun N/A:N/A (2018). Read more (PubMed: 29961753) »
See all 9 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your enquiry.

Unfortunately we are not aware of any publication using the ab88523. This does not mean that this antibody has not been used in a publication, it just means that we do not know about it.

I can however confirm that the laboratory has tested this antibody in immunohistochemistry on paraffin embedded sections and on human tissue. We do therefore guarantee this antibody in this application with our Abpromise guarantee (https://www.abcam.com/abpromise).

Should you need the antibody for staining in frozen section or on mouse tissue for example, please do contact me again with the details of your planned experiment, as I might be able to provide you with a testing discount.

I hope this information is helpful. Please do not hesitate to contact us again should you have any further questions.

I wish you a good evening!

Read More

Answer

Thank you for your reply.
I'm sending a free of charge vial of ab14694 on the order ***, which should arrive on Monday. Please keep me updated about the performance of this replacement antibody.
I look forward to hearing from you. Please let me know if there is anything else you need from me.

Read More

Answer

Thank you for your reply and for providing so many details about the protocol. This information will be helpful for our investigation of this antibody.
I've found an order going to ***. Can you confirm that this is your order? I can send the replacement antibody, ab14694, free of charge if you would like to try it, and if it still does not work then we would be happy to issue a credit or refund.
I look forward to hearing from you and resolving this promptly. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

Answer

According to our records, ab88523 was proving difficult to use in Western blot and we were in contact in order to help resolve the issue.
Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.
If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.
We wish you the best of luck with your research and look forward to a reply.

Read More

Answer

Thank you for contacting us and letting us know about the trouble with this antibody.
I do have a couple of additional questions, so that I can better understand the situation:
1) Do you know your order number or lot number?
2) How has the antibody been stored?
3) What solution do you use for blocking the membrane (BSA or milk)?
4) How were the lysates prepared (reduced, denatured, protease inhibitors used)?
5) How much protein was loaded onto the gel per lane?
6) On the image that you sent, what are the top two molecular weight markers? I'm not sure if these are 40 and 50 kDa?
7) On the image, what is the difference between the lanes? Are these different lysates, and if so which is in each lane?
8) Were any of the cells treated in order to induce tetherin expression, or are you analyzing endogenous levels?
According to SwissProt, the tetherin primary amino acid sequence is cleaved to produce the mature protein, which removes 19 amino acids. This mature protein corresponds to about 18 kDa, which is close to the lower band on the image. However this mature protein is generally glycosylated and is seen by WB around 30 kDa using ab88523. At this point I'm not sure if the lower band on the image corresponds to tetherin.
We do guarantee all of our products to work as stated on the datasheet for up to 6 months after purchase, so I would be happy to send a replacement or alternatively issue a credit or refund. We also have ab14694, which as been cited in two references-
https://www.abcam.com/BST2-antibody-ab14694.html
I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that I can do for you.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up