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I recently ordered an anti-tetherin antibody (ab88523) and tried it in a western blot last week. There are a number of non-specific bands and no band at the size stated in the datasheet. There is a band at lower size but I am not sure if this is correct. The cell lines used were Vero and two types of Hela cells, the Hela particularly should express large amounts of tetherin. I used the antibody at 1 in 200 overnight at 4 degrees. I have attached a picture of the blot and any advice you could give me would be appreciated.
Asked on Apr 17 2012
According to our records, ab88523 was proving difficult to use in Western blot and we were in contact in order to help resolve the issue.
Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.
If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.
We wish you the best of luck with your research and look forward to a reply.
Answered on Apr 17 2012