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Apologies for my delay in replying!!
In answer to your questions:
1. Lot number ***
2. Antibody in stored at -20 and was used within a week of delivery
5. Approximately 10ug
6. Yes they are 40 and 50kDa
7. Yes they are different lysates
Lane 1. Vero cells
Lane 2. Hela cells
Lane 3. Hela cells (a new batch)
We suspect the Hela will have more BST-2 than the Vero, certainly Hela do express BST-2 according to the literature.
8. All endogenous levels
I am happy to try the other antibody you suggest with our lysates, if we get a refund on this one of course!!
Many thanks for your help,
Asked on Apr 18 2012
Thank you for your reply and for providing so many details about the protocol. This information will be helpful for our investigation of this antibody.
I've found an order going to ***. Can you confirm that this is your order? I can send the replacement antibody, ab14694, free of charge if you would like to try it, and if it still does not work then we would be happy to issue a credit or refund.
I look forward to hearing from you and resolving this promptly. Please let me know if you have any questions or if there is anything else that we can do for you.
Answered on Apr 18 2012