Recombinant Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)
![Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)](https://www.abcam.com/ps/products/243/ab243229/Images/ab243229-333314-anti-bst2-antibody-epr20202-169-immunoprecipitation.jpg "Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20202-169] to BST2/Tetherin
- Suitable for: WB, IP, Flow Cyt, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BST2/Tetherin antibody [EPR20202-169]
See all BST2/Tetherin primary antibodies -
Description
Rabbit monoclonal [EPR20202-169] to BST2/Tetherin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, whole cell lysate, in the loading buffer containing DTT; K562 whole cell lysate, in the loading buffer containing DTT; U-937 whole cell lysate. ICC/IF: HeLa and U-937 cells. Flow Cyt: HeLa and U-937 cells. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20202-169 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 35, 70 kDa (predicted molecular weight: 20 kDa).
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| IP |
1/30.
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| Flow Cyt |
1/500.
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| ICC/IF |
1/100.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 35, 70 kDa (predicted molecular weight: 20 kDa). |
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IP
1/30. |
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Flow Cyt
1/500. |
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ICC/IF
1/100. |
Target
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Function
May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis. -
Tissue specificity
Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level). -
Sequence similarities
Belongs to the tetherin family. -
Domain
The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading. -
Post-translational
modificationsMonoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation. -
Cellular localization
Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein. - Information by UniProt
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Database links
- Entrez Gene: 684 Human
- Omim: 600534 Human
- SwissProt: Q10589 Human
- Unigene: 118110 Human
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Alternative names
- Bone marrow stromal antigen 2 antibody
- Bone marrow stromal cell antigen 2 antibody
- Bone marrow stromal cell antigen antibody
see all
Images
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Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)
BST2/Tetherin was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243229 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab243229 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 µg (input).
Lane 2: ab243229 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243229 in HeLa whole cell lysate.Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 seconds. -
![Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)](https://www.abcam.com/ps/products/243/ab243229/Images/ab243229-333313-anti-bst2-antibody-epr20202-169-flow-cytometry.jpg)
Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)Flow cytometric analysis of U-937 (human histiocytic lymphoma cell line) cell line labeling BST2/Tetherin with ab243229 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)](https://www.abcam.com/ps/products/243/ab243229/Images/ab243229-333312-anti-bst2-antibody-epr20202-169-flow-cytometry.jpg)
Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling BST2/Tetherin with ab243229 at 1/500 dilution (Red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)](https://www.abcam.com/ps/products/243/ab243229/Images/ab243229-333311-anti-bst2-antibody-epr20202-169-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-937 (human histiocytic lymphoma cell line) cells labeling BST2/Tetherin with ab243229 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in U-937 cells (PMID: 20529266).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)](https://www.abcam.com/ps/products/243/ab243229/Images/ab243229-333310-anti-bst2-antibody-epr20202-169-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling BST2/Tetherin with ab243229 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cells (PMID: 20529266).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Western blot - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)](https://www.abcam.com/ps/products/243/ab243229/Images/ab243229-333309-anti-bst2-antibody-epr20202-169-western-blot.jpg)
Western blot - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229) at 1/1000 dilution + U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 20 kDa
Observed band size: 35,70 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19737401)
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![Western blot - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)](https://www.abcam.com/ps/products/243/ab243229/Images/ab243229-333308-anti-bst2-antibody-epr20202-169-western-blot.jpg)
Western blot - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)All lanes : Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinom), whole cell lysate, in the loading buffer containing DTT
Lane 2 : HeLa whole cell lysate in the loading buffer without DTT
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate, in the loading buffer containing DTT
Lane 4 : K562 whole cell lysate in the loading buffer without DTT
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 20 kDa
Observed band size: 35,70 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19196977; PMID: 19737401).
BST2/Tetherin is a glycosylated protein, its calculated MW is 20kDa, and the observed MW is 35 kDa, which is consistent to the literature.
Both 35 and 70-kDa bands were detected under the reducing condition, whereas under the non-reducing condition, only the 70-kDa band was detected.
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![Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)](https://www.abcam.com/ps/products/243/ab243229/Images/ab243229-8-benefits-of-recombinant-antibodies.png)
Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab243229 has been referenced in 1 publication.
- Ding Y et al. Parkinson's Disease Causative Mutation in Vps35 Disturbs Tetherin Trafficking to Cell Surfaces and Facilitates Virus Spread. Cells 10:N/A (2021). PubMed: 33800686