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    bst2tetherin-antibody-epr23597-266-ab272169.pdf

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RecombinantRabMAb

Recombinant Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
  • Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
  • Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23597-266] to BST2/Tetherin
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
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Anti-SLC28A1 antibody (ab192438)

View more associated products

Overview

  • Product name

    Anti-BST2/Tetherin antibody [EPR23597-266]
    See all BST2/Tetherin primary antibodies
  • Description

    Rabbit monoclonal [EPR23597-266] to BST2/Tetherin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse spleen, thymus and lymph node tissue lysates; P388D1 whole cell lysate. ICC/IF: Mouse splenocytes. Flow Cyt: Mouse splenocytes. IP: Mouse spleen tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23597-266
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-BST2/Tetherin antibody [EPR23597-266] - BSA and Azide free (ab272176)
    • Alexa Fluor® 488 Anti-BST2/Tetherin antibody [EPR23597-266] (ab275192)
    • Alexa Fluor® 647 Anti-BST2/Tetherin antibody [EPR23597-266] (ab275193)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 20 kDa.
ICC/IF
1/100.
Flow Cyt
1/500.
IP
1/30.
Notes
WB
1/1000. Predicted molecular weight: 20 kDa.
ICC/IF
1/100.
Flow Cyt
1/500.
IP
1/30.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis.
  • Tissue specificity

    Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level).
  • Sequence similarities

    Belongs to the tetherin family.
  • Domain

    The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading.
  • Post-translational
    modifications

    Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation.
  • Cellular localization

    Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein.
  • Target information above from: UniProt accession Q10589 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 69550 Mouse
    • SwissProt: Q8R2Q8 Mouse
    • Unigene: 260325 Mouse
    • Alternative names

      • Bone marrow stromal antigen 2 antibody
      • Bone marrow stromal cell antigen 2 antibody
      • Bone marrow stromal cell antigen antibody
      • BST 2 antibody
      • BST-2 antibody
      • BST2 antibody
      • BST2_HUMAN antibody
      • CD 317 antibody
      • CD317 antibody
      • CD317 antigen antibody
      • HM1.24 antigen antibody
      • NPC A 7 antibody
      • Tetherin antibody
      see all

    Images

    • Western blot - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
      Western blot - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
      All lanes : Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169) at 1/1000 dilution

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : Mouse thymus tissue lysate
      Lane 3 : Mouse lymph node tissue lysate
      Lane 4 : P388D1 (mouse lymphoma monocyte; macrophage), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 20 kDa
      Observed band size: 28-40,64 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      BST2 is type II transmembrane glycoprotein with a molecular mass of 28-40 KD, and forms cysteine-linked dimers at 64 KD which is consistent to the literature(PMID: 22520941; PMID: 19737401).

    • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
      Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Mouse splenocyte cells labelling BST2/Tetherin with ab272169 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic staining in mouse splenocytes. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

    • Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
      Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)

      Flow cytometric analysis of Mouse splenocyte cells labelling BST2/Tetherin with ab272169 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (AlexaFluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG (Left) or ab272169 (Right). Then stained with anti-CD45R conjugated to Alexa Fluor® 647.

      Gated on viable cells.

       

    • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
      Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)

      BST2/Tetherin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab272169 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272169 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse spleen tissue lysate 10ug

      Lane 2: ab272169 IP in Mouse spleen tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272169 in mouse spleen tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 15 seconds

      BST2 is type II transmembrane glycoprotein with a molecular mass of 28-40 KD, which is consistent to the literature(PMID: 22520941; PMID: 19737401).

    • Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
      Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab272169? Please let us know so that we can cite the reference in this datasheet.

    ab272169 has not yet been referenced specifically in any publications.

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