Recombinant Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
![Western blot - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)](https://www.abcam.com/ps/products/272/ab272169/Images/ab272169-387023-anti-bst2tetherin-antibody-epr23597266-western-blot-mouse-spleen-mouse-thymusmouse-lymph-node-p388d1-lysates.jpg "Western blot - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23597-266] to BST2/Tetherin
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-BST2/Tetherin antibody [EPR23597-266]
See all BST2/Tetherin primary antibodies -
Description
Rabbit monoclonal [EPR23597-266] to BST2/Tetherin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse spleen, thymus and lymph node tissue lysates; P388D1 whole cell lysate. ICC/IF: Mouse splenocytes. Flow Cyt: Mouse splenocytes. IP: Mouse spleen tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23597-266 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 20 kDa.
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| ICC/IF |
1/100.
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| Flow Cyt |
1/500.
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| IP |
1/30.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 20 kDa. |
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ICC/IF
1/100. |
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Flow Cyt
1/500. |
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IP
1/30. |
Target
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Function
May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis. -
Tissue specificity
Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level). -
Sequence similarities
Belongs to the tetherin family. -
Domain
The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading. -
Post-translational
modificationsMonoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation. -
Cellular localization
Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein. - Information by UniProt
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Database links
- Entrez Gene: 69550 Mouse
- SwissProt: Q8R2Q8 Mouse
- Unigene: 260325 Mouse
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Alternative names
- Bone marrow stromal antigen 2 antibody
- Bone marrow stromal cell antigen 2 antibody
- Bone marrow stromal cell antigen antibody
see all
Images
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Western blot - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)All lanes : Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse thymus tissue lysate
Lane 3 : Mouse lymph node tissue lysate
Lane 4 : P388D1 (mouse lymphoma monocyte; macrophage), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 20 kDa
Observed band size: 28-40,64 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
BST2 is type II transmembrane glycoprotein with a molecular mass of 28-40 KD, and forms cysteine-linked dimers at 64 KD which is consistent to the literature(PMID: 22520941; PMID: 19737401).
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![Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)](https://www.abcam.com/ps/products/272/ab272169/Images/ab272169-1-anti-bst2tetherin-antibody-epr23597266-immunocytochemistry-mouse-splenocytes.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Mouse splenocyte cells labelling BST2/Tetherin with ab272169 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic staining in mouse splenocytes. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.
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![Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)](https://www.abcam.com/ps/products/272/ab272169/Images/ab272169-2-anti-bst2tetherin-antibody-epr23597266-flow-cytometry-mouse-splenocytes.jpg)
Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)Flow cytometric analysis of Mouse splenocyte cells labelling BST2/Tetherin with ab272169 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (AlexaFluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab272169 (Right). Then stained with anti-CD45R conjugated to Alexa Fluor® 647.
Gated on viable cells.
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![Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)](https://www.abcam.com/ps/products/272/ab272169/Images/ab272169-3-anti-bst2tetherin-antibody-epr23597266-immunoprecipitation-mouse-spleen-tissue-lysate-10ug.jpg)
Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)BST2/Tetherin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab272169 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272169 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse spleen tissue lysate 10ug
Lane 2: ab272169 IP in Mouse spleen tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272169 in mouse spleen tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds
BST2 is type II transmembrane glycoprotein with a molecular mass of 28-40 KD, which is consistent to the literature(PMID: 22520941; PMID: 19737401).
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![Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)](https://www.abcam.com/ps/products/272/ab272169/Images/ab272169-5-benefits-of-recombinant-antibodies.png)
Anti-BST2/Tetherin antibody [EPR23597-266] (ab272169)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab272169 has not yet been referenced specifically in any publications.