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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-BST2/Tetherin antibody [EPR5648] (ab134061)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (5)

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Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
  • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
  • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
  • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
  • Anti-BST2/Tetherin antibody [EPR5648] (ab134061)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5648] to BST2/Tetherin
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human BST2/Tetherin protein (ab114390)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human BST2 (Tetherin) knockout HeLa cell line (ab265527)

View more associated products

Overview

  • Product name

    Anti-BST2/Tetherin antibody [EPR5648]
    See all BST2/Tetherin primary antibodies
  • Description

    Rabbit monoclonal [EPR5648] to BST2/Tetherin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human BST2/Tetherin aa 100-200 (extracellular).
    Database link: Q10589

  • Positive control

    • WB: A431, U937, Molt-4, Human spleen and HeLa lysates; Human kidney tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as BST2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.18 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5648
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Associated products

  • Alternative Versions

    • Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free (ab232454)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human BST2 (Tetherin) knockout A-431 cell line (ab261907)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human BST2/Tetherin protein (ab114390)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis.
    • Tissue specificity

      Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level).
    • Sequence similarities

      Belongs to the tetherin family.
    • Domain

      The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading.
    • Post-translational
      modifications

      Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation.
    • Cellular localization

      Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein.
    • Target information above from: UniProt accession Q10589 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 684 Human
      • Omim: 600534 Human
      • SwissProt: Q10589 Human
      • Unigene: 118110 Human
      • Alternative names

        • Bone marrow stromal antigen 2 antibody
        • Bone marrow stromal cell antigen 2 antibody
        • Bone marrow stromal cell antigen antibody
        • BST 2 antibody
        • BST-2 antibody
        • BST2 antibody
        • BST2_HUMAN antibody
        • CD 317 antibody
        • CD317 antibody
        • CD317 antigen antibody
        • HM1.24 antigen antibody
        • NPC A 7 antibody
        • Tetherin antibody
        see all

      Images

      • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
        Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
        All lanes : Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

        Lane 1 : Wild-type A431 cell lysate
        Lane 2 : BST2 knockout A431 cell lysate
        Lane 3 : HeLa cell lysate
        Lane 4 : SH-SY5Y cell lysate

        Lysates/proteins at 40 µg per lane.

        Performed under reducing conditions.

        Predicted band size: 20 kDa
        Observed band size: 30 kDa why is the actual band size different from the predicted?


        Exposure time: 20 minutes


        ab134061 was shown to react with BST2 in A431 wild-type cells in western blot. Loss of signal was observed when BST2 knockout sample was used. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134061 overnight at 4°C at a 1 in 1000 dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor® 680) at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.

      • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
        Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
        Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

        Predicted band size: 20 kDa
        Observed band size: 28-40 kDa why is the actual band size different from the predicted?

      • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
        Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
        Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution (Purified) + Human fetal spleen whole cell lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 20 kDa
        Observed band size: 30 kDa why is the actual band size different from the predicted?


        Exposure time: 3 minutes


        Blocking/Diluting buffer and concentration 5% NFDM/TBST

        This antibody detects a non-specific band at 65 KDa.

        This image was produced using unpurified antibody.

      • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
        Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
        All lanes : Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

        Lane 1 : U937 lysate
        Lane 2 : Molt-4 lysate
        Lane 3 : Human spleen lysate
        Lane 4 : HeLa lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 20 kDa



        This image was produced using unpurified antibody.

      • Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
        Anti-BST2/Tetherin antibody [EPR5648] (ab134061)

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (5)

      Publishing research using ab134061? Please let us know so that we can cite the reference in this datasheet.

      ab134061 has been referenced in 5 publications.

      • Zhang G  et al. CD317 Activates EGFR by Regulating Its Association with Lipid Rafts. Cancer Res 79:2220-2231 (2019). PubMed: 30890618
      • Balzano M  et al. Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche. Cell Rep 26:3257-3271.e8 (2019). PubMed: 30893599
      • Dufrasne FE  et al. Modulation of the NF-?B signaling pathway by the HIV-2 envelope glycoprotein and its incomplete BST-2 antagonism. Virology 513:11-16 (2018). WB . PubMed: 29028477
      • Sithole N  et al. DDX17 Specifically, and Independently of DDX5, Controls Use of the HIV A4/5 Splice Acceptor Cluster and Is Essential for Efficient Replication of HIV. J Mol Biol 430:3111-3128 (2018). PubMed: 30131116
      • Li X  et al. CD317 Promotes the survival of cancer cells through apoptosis-inducing factor. J Exp Clin Cancer Res 35:117 (2016). PubMed: 27444183

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-BST2/Tetherin antibody [EPR5648]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (RERF-LC-Ad1 cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      25 µg
      Specification
      RERF-LC-Ad1 cells
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 06 2017

      Immunocytochemistry/ Immunofluorescence abreview for Anti-BST2/Tetherin antibody [EPR5648]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HEK 293T, HFF, HFtelo, Hela etc)
      Permeabilization
      Yes - 0.1% TX-100, 0.01% SDS in 1xPBS
      Specification
      HEK 293T, HFF, HFtelo, Hela etc
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 20 2017

      Flow Cytometry abreview for Anti-BST2/Tetherin antibody [EPR5648]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (HEK293T, HFF, HFtelo etc)
      Permeabilization
      No
      Gating Strategy
      BST-2-(+) cell
      Specification
      HEK293T, HFF, HFtelo etc
      Preparation
      Cell harvesting/tissue preparation method: using T/E
      Sample buffer: 1% FBS in 1xPBS (filterated)
      Fixation
      Formaldehyde
      Read More

      Miss. Kim JEONG-JIN

      Verified customer

      Submitted Feb 20 2017

      Immunoprecipitation abreview for Anti-BST2/Tetherin antibody [EPR5648]

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      Human Cell lysate - whole cell (human embryonic Kedney 293 T)
      Total protein in input
      2.5e+006 cells
      Immuno-precipitation step
      Protein A
      Specification
      human embryonic Kedney 293 T
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 20 2017

      Western blot abreview for Anti-BST2/Tetherin antibody [EPR5648]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (whole cell type)
      Gel Running Conditions
      Reduced Denaturing (gel 12%)
      Loading amount
      1 µg
      Treatment
      Type I IFN (1000unit/ml) for different times, Herpes virus infection (MOI: several amounts) for different times
      Specification
      whole cell type
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Miss. Kim JEONG-JIN

      Verified customer

      Submitted Feb 20 2017

      Question

      Would it be possible to know the general aa range from which the sequence used to generate 3885-1 is taken?

      Also, would it be possible to know the concentration from lot YI010412?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 08 2013

      Answer

      The immunogen sequence is within the range of amino acids 100 - 150 of the sequence on the following UniProt page.

      http://www.uniprot.org/uniprot/Q10589

      The concentration of lot YI010412 is 0.11mg/ml.

      Read More

      Abcam Scientific Support

      Answered on Feb 08 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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