Recombinant Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5648] to BST2/Tetherin
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-BST2/Tetherin antibody [EPR5648]
See all BST2/Tetherin primary antibodies -
Description
Rabbit monoclonal [EPR5648] to BST2/Tetherin -
Host species
Rabbit -
Specificity
For Mouse and Rat species, we have preliminary internal testing data to indicate this antibody may not react. Please contact us for more information.
-
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human BST2/Tetherin aa 100-200 (extracellular).
Database link: Q10589 -
Positive control
- WB: A431, U937, Molt-4, Human spleen and HeLa lysates; Human kidney tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.18 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5648 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
KO cell lysates
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
Target
-
Function
May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis. -
Tissue specificity
Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level). -
Sequence similarities
Belongs to the tetherin family. -
Domain
The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading. -
Post-translational
modificationsMonoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation. -
Cellular localization
Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein. - Information by UniProt
-
Database links
- Entrez Gene: 684 Human
- Omim: 600534 Human
- SwissProt: Q10589 Human
- Unigene: 118110 Human
-
Alternative names
- Bone marrow stromal antigen 2 antibody
- Bone marrow stromal cell antigen 2 antibody
- Bone marrow stromal cell antigen antibody
see all
Images
-
All lanes : Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : BST2 knockout A431 cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesab134061 was shown to react with BST2 in A431 wild-type cells in western blot. Loss of signal was observed when BST2 knockout sample was used. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134061 overnight at 4°C at a 1 in 1000 dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor® 680) at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.
-
Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 28-40 kDa why is the actual band size different from the predicted? -
Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution (Purified) + Human fetal spleen whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Diluting buffer and concentration 5% NFDM/TBST
This antibody detects a non-specific band at 65 KDa.
This image was produced using unpurified antibody.
-
All lanes : Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution
Lane 1 : U937 lysate
Lane 2 : Molt-4 lysate
Lane 3 : Human spleen lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 20 kDaThis image was produced using unpurified antibody.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab134061 has been referenced in 6 publications.
- Ding Y et al. Parkinson's Disease Causative Mutation in Vps35 Disturbs Tetherin Trafficking to Cell Surfaces and Facilitates Virus Spread. Cells 10:N/A (2021). PubMed: 33800686
- Zhang G et al. CD317 Activates EGFR by Regulating Its Association with Lipid Rafts. Cancer Res 79:2220-2231 (2019). PubMed: 30890618
- Balzano M et al. Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche. Cell Rep 26:3257-3271.e8 (2019). PubMed: 30893599
- Dufrasne FE et al. Modulation of the NF-?B signaling pathway by the HIV-2 envelope glycoprotein and its incomplete BST-2 antagonism. Virology 513:11-16 (2018). WB . PubMed: 29028477
- Sithole N et al. DDX17 Specifically, and Independently of DDX5, Controls Use of the HIV A4/5 Splice Acceptor Cluster and Is Essential for Efficient Replication of HIV. J Mol Biol 430:3111-3128 (2018). PubMed: 30131116
- Li X et al. CD317 Promotes the survival of cancer cells through apoptosis-inducing factor. J Exp Clin Cancer Res 35:117 (2016). PubMed: 27444183