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  1. Link

    bst2tetherin-antibody-epr5648-ab134061.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-BST2/Tetherin antibody [EPR5648] (ab134061)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (6)

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Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
  • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
  • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
  • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
  • Anti-BST2/Tetherin antibody [EPR5648] (ab134061)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5648] to BST2/Tetherin
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human BST2/Tetherin protein (ab114390)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human BST2 (Tetherin) knockout A-431 cell line (ab261907)

View more associated products

Overview

  • Product name

    Anti-BST2/Tetherin antibody [EPR5648]
    See all BST2/Tetherin primary antibodies
  • Description

    Rabbit monoclonal [EPR5648] to BST2/Tetherin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human BST2/Tetherin aa 100-200 (extracellular).
    Database link: Q10589

  • Positive control

    • WB: A431, U937, Molt-4, Human spleen and HeLa lysates; Human kidney tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.18 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5648
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free (ab232454)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human BST2 (Tetherin) knockout A-431 cell line (ab261907)
  • KO cell lysates

    • Human BST2 (Tetherin) knockout A-431 cell lysate (ab261713)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human BST2/Tetherin protein (ab114390)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
Application notes
Is unsuitable for ICC/IF or IHC-P.

Target

  • Function

    May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis.
  • Tissue specificity

    Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level).
  • Sequence similarities

    Belongs to the tetherin family.
  • Domain

    The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading.
  • Post-translational
    modifications

    Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation.
  • Cellular localization

    Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein.
  • Target information above from: UniProt accession Q10589 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 684 Human
    • Omim: 600534 Human
    • SwissProt: Q10589 Human
    • Unigene: 118110 Human
    • Alternative names

      • Bone marrow stromal antigen 2 antibody
      • Bone marrow stromal cell antigen 2 antibody
      • Bone marrow stromal cell antigen antibody
      • BST 2 antibody
      • BST-2 antibody
      • BST2 antibody
      • BST2_HUMAN antibody
      • CD 317 antibody
      • CD317 antibody
      • CD317 antigen antibody
      • HM1.24 antigen antibody
      • NPC A 7 antibody
      • Tetherin antibody
      see all

    Images

    • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
      Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
      All lanes : Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

      Lane 1 : Wild-type A431 cell lysate
      Lane 2 : BST2 knockout A431 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 40 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 20 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?


      Exposure time: 20 minutes


      ab134061 was shown to react with BST2 in A431 wild-type cells in western blot. Loss of signal was observed when BST2 knockout sample was used. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134061 overnight at 4°C at a 1 in 1000 dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor® 680) at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.

    • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
      Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
      Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 20 kDa
      Observed band size: 28-40 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
      Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
      Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution (Purified) + Human fetal spleen whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 20 kDa
      Observed band size: 30 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Diluting buffer and concentration 5% NFDM/TBST

      This antibody detects a non-specific band at 65 KDa.

      This image was produced using unpurified antibody.

    • Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
      Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
      All lanes : Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

      Lane 1 : U937 lysate
      Lane 2 : Molt-4 lysate
      Lane 3 : Human spleen lysate
      Lane 4 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 20 kDa



      This image was produced using unpurified antibody.

    • Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
      Anti-BST2/Tetherin antibody [EPR5648] (ab134061)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab134061? Please let us know so that we can cite the reference in this datasheet.

    ab134061 has been referenced in 6 publications.

    • Ding Y  et al. Parkinson's Disease Causative Mutation in Vps35 Disturbs Tetherin Trafficking to Cell Surfaces and Facilitates Virus Spread. Cells 10:N/A (2021). PubMed: 33800686
    • Zhang G  et al. CD317 Activates EGFR by Regulating Its Association with Lipid Rafts. Cancer Res 79:2220-2231 (2019). PubMed: 30890618
    • Balzano M  et al. Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche. Cell Rep 26:3257-3271.e8 (2019). PubMed: 30893599
    • Dufrasne FE  et al. Modulation of the NF-?B signaling pathway by the HIV-2 envelope glycoprotein and its incomplete BST-2 antagonism. Virology 513:11-16 (2018). WB . PubMed: 29028477
    • Sithole N  et al. DDX17 Specifically, and Independently of DDX5, Controls Use of the HIV A4/5 Splice Acceptor Cluster and Is Essential for Efficient Replication of HIV. J Mol Biol 430:3111-3128 (2018). PubMed: 30131116
    • Li X  et al. CD317 Promotes the survival of cancer cells through apoptosis-inducing factor. J Exp Clin Cancer Res 35:117 (2016). PubMed: 27444183

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-5 of 5 Abreviews

    Western blot abreview for Anti-BST2/Tetherin antibody [EPR5648]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (RERF-LC-Ad1 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    RERF-LC-Ad1 cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 06 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-BST2/Tetherin antibody [EPR5648]

    Poor
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK 293T, HFF, HFtelo, Hela etc)
    Permeabilization
    Yes - 0.1% TX-100, 0.01% SDS in 1xPBS
    Specification
    HEK 293T, HFF, HFtelo, Hela etc
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 20 2017

    Flow Cytometry abreview for Anti-BST2/Tetherin antibody [EPR5648]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (HEK293T, HFF, HFtelo etc)
    Permeabilization
    No
    Gating Strategy
    BST-2-(+) cell
    Specification
    HEK293T, HFF, HFtelo etc
    Preparation
    Cell harvesting/tissue preparation method: using T/E
    Sample buffer: 1% FBS in 1xPBS (filterated)
    Fixation
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Kim JEONG-JIN

    Verified customer

    Submitted Feb 20 2017

    Immunoprecipitation abreview for Anti-BST2/Tetherin antibody [EPR5648]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (human embryonic Kedney 293 T)
    Total protein in input
    2.5e+006 cells
    Immuno-precipitation step
    Protein A
    Specification
    human embryonic Kedney 293 T
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 20 2017

    Western blot abreview for Anti-BST2/Tetherin antibody [EPR5648]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (whole cell type)
    Gel Running Conditions
    Reduced Denaturing (gel 12%)
    Loading amount
    1 µg
    Treatment
    Type I IFN (1000unit/ml) for different times, Herpes virus infection (MOI: several amounts) for different times
    Specification
    whole cell type
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Kim JEONG-JIN

    Verified customer

    Submitted Feb 20 2017

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