Recombinant Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
![Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)](https://www.abcam.com/ps/products/134/ab134061/Images/ab134061-412979-anti-bst2tetherin-antibody-epr5648-western-blot-a431-wt-a431-bst2-knockout-hela-shsy5y.jpg "Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5648] to BST2/Tetherin
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BST2/Tetherin antibody [EPR5648]
See all BST2/Tetherin primary antibodies -
Description
Rabbit monoclonal [EPR5648] to BST2/Tetherin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human BST2/Tetherin aa 100-200 (extracellular).
Database link: Q10589 -
Positive control
- WB: A431, U937, Molt-4, Human spleen and HeLa lysates; Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.18 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5648 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
Target
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Function
May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis. -
Tissue specificity
Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level). -
Sequence similarities
Belongs to the tetherin family. -
Domain
The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading. -
Post-translational
modificationsMonoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation. -
Cellular localization
Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein. - Information by UniProt
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Database links
- Entrez Gene: 684 Human
- Omim: 600534 Human
- SwissProt: Q10589 Human
- Unigene: 118110 Human
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Alternative names
- Bone marrow stromal antigen 2 antibody
- Bone marrow stromal cell antigen 2 antibody
- Bone marrow stromal cell antigen antibody
see all
Images
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Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)All lanes : Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : BST2 knockout A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesab134061 was shown to react with BST2 in A431 wild-type cells in western blot. Loss of signal was observed when BST2 knockout sample was used. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134061 overnight at 4°C at a 1 in 1000 dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor® 680) at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.
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![Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)](https://www.abcam.com/ps/products/134/ab134061/Images/ab134061-307069-anti-bst2-antibody-epr5648-western-blot.jpg)
Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 20 kDa
Observed band size: 28-40 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)](https://www.abcam.com/ps/products/134/ab134061/Images/ab134061-293051-anti-bst2-antibody-epr5648-western-blot.jpg)
Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution (Purified) + Human fetal spleen whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Diluting buffer and concentration 5% NFDM/TBST
This antibody detects a non-specific band at 65 KDa.
This image was produced using unpurified antibody.
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![Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)](https://www.abcam.com/ps/products/134/ab134061/Images/ab134061-148181-anti-bst2-antibody-epr5648-western-blot.jpg)
Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061)All lanes : Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution
Lane 1 : U937 lysate
Lane 2 : Molt-4 lysate
Lane 3 : Human spleen lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 20 kDaThis image was produced using unpurified antibody.
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![Anti-BST2/Tetherin antibody [EPR5648] (ab134061)](https://www.abcam.com/ps/products/134/ab134061/Images/ab134061-7-benefits-of-recombinant-antibodies.png)
Anti-BST2/Tetherin antibody [EPR5648] (ab134061)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab134061 has been referenced in 6 publications.
- Ding Y et al. Parkinson's Disease Causative Mutation in Vps35 Disturbs Tetherin Trafficking to Cell Surfaces and Facilitates Virus Spread. Cells 10:N/A (2021). PubMed: 33800686
- Zhang G et al. CD317 Activates EGFR by Regulating Its Association with Lipid Rafts. Cancer Res 79:2220-2231 (2019). PubMed: 30890618
- Balzano M et al. Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche. Cell Rep 26:3257-3271.e8 (2019). PubMed: 30893599
- Dufrasne FE et al. Modulation of the NF-?B signaling pathway by the HIV-2 envelope glycoprotein and its incomplete BST-2 antagonism. Virology 513:11-16 (2018). WB . PubMed: 29028477
- Sithole N et al. DDX17 Specifically, and Independently of DDX5, Controls Use of the HIV A4/5 Splice Acceptor Cluster and Is Essential for Efficient Replication of HIV. J Mol Biol 430:3111-3128 (2018). PubMed: 30131116
- Li X et al. CD317 Promotes the survival of cancer cells through apoptosis-inducing factor. J Exp Clin Cancer Res 35:117 (2016). PubMed: 27444183