Key features and details
- Rabbit polyclonal to BTAF1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BTAF1 antibody
See all BTAF1 primary antibodies
DescriptionRabbit polyclonal to BTAF1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey
Synthetic peptide corresponding to Human BTAF1 aa 75-125.
Database link: NP_003963.1
- Whole cell lysates from HeLa and 293T cells.
Previously labelled as TAF172.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRegulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 8 HEAT repeats.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
- Information by UniProt
- ATP dependent helicase BTAF1 antibody
- ATP-dependent helicase BTAF1 antibody
- B-TFIID transcription factor associated 170 kDa subunit antibody
All lanes : Anti-BTAF1 antibody (ab72285) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 207 kDa
Observed band size: 207 kDa
Additional bands at: 170 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Detection of Human BTAF1 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab72285 at 3µg/mg lysate for IP and at 1µg/ml for subsequent WB.
ab72285 has been referenced in 1 publication.
- Xue Y et al. Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals. Mol Cell 67:594-607.e4 (2017). PubMed: 28735899