• Product name

  • Description

    Rabbit polyclonal to BTAF1
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human BTAF1 aa 75-125.
    Database link: NP_003963.1

  • Positive control

    • Whole cell lysates from HeLa and 293T cells.
  • General notes

    Previously labelled as TAF172.



Our Abpromise guarantee covers the use of ab72285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function

    Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family.
    Contains 8 HEAT repeats.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent helicase BTAF1 antibody
    • ATP-dependent helicase BTAF1 antibody
    • B-TFIID transcription factor associated 170 kDa subunit antibody
    • B-TFIID transcription factor associated, 170kDa (Mot1 homolog, S. cerevisiae) antibody
    • B-TFIID transcription factor-associated 170 kDa subunit antibody
    • BTAF 1 RNA polymerase II antibody
    • BTAF1 antibody
    • BTAF1 RNA polymerase II B TFIID transcription factor associated 170kDa antibody
    • BTAF1_HUMAN antibody
    • MOT 1 antibody
    • MOT1 antibody
    • MOT1 Yeast homolog of antibody
    • TAF 172 antibody
    • TAF II 170 antibody
    • TAF(II)170 antibody
    • TAF-172 antibody
    • TAF172 antibody
    • TAFII170 antibody
    • TATA binding protein associated factor 172 antibody
    • TATA-binding protein-associated factor 172 antibody
    • TBP associated factor 172 antibody
    • TBP-associated factor 172 antibody
    see all


  • All lanes : Anti-BTAF1 antibody (ab72285) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 207 kDa
    Observed band size: 207 kDa
    Additional bands at: 170 kDa, 85 kDa. We are unsure as to the identity of these extra bands.

  • Detection of Human BTAF1 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab72285 at 3µg/mg lysate for IP and at 1µg/ml for subsequent WB.


This product has been referenced in:

  • Xue Y  et al. Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals. Mol Cell 67:594-607.e4 (2017). Read more (PubMed: 28735899) »
See 1 Publication for this product

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