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Rabbit polyclonal to BTBD15
Predicted to work with:
Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
Synthetic peptide, corresponding to a region within amino acids 300-350 of Human BTBD15 (BAI46608.1).
Jurkat, HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7-8
Concentration information loading...
Immunogen affinity purified
ab117775 was affinity purified using an epitope specific to BTBD15 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 64 kDa.
Use at 2-10 µg/mg of lysate.
May be involved in transcriptional regulation.
Contains 1 BTB (POZ) domain.
Contains 4 C2H2-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
BTB (POZ) domain containing 15 antibody
BTB/POZ domain-containing protein 15 antibody
Western blot - Anti-BTBD15 antibody (ab117775)
All lanes : Anti-BTBD15 antibody (ab117775) at 0.1 µg/ml Lane 1 : Jurkat whole cell lysate at 50 µg Lane 2 : Jurkat whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 50 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 64 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-BTBD15 antibody (ab117775)
Detection of BTBD15 by Western Blot of Immunprecipitate.
ab117775 at 1µg/ml staining BTBD15 in Jurkat whole cell lysate immunoprecipitated using ab117775 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"