Recombinant Anti-BTBD17 antibody [EPR9593(B)] (ab138507)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9593(B)] to BTBD17
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-BTBD17 antibody [EPR9593(B)]
See all BTBD17 primary antibodies -
Description
Rabbit monoclonal [EPR9593(B)] to BTBD17 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BTBD17 aa 1-100. The exact sequence is proprietary.
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Positive control
- Human colon cancer, Human fetal kidney, and Human bladder lysates, Human kidney clear cell carcinoma and Human liver tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9593(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab138507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/10000. Predicted molecular weight: 52 kDa. | |
IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Sequence similarities
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 388419 Human
- SwissProt: A6NE02 Human
- Unigene: 211870 Human
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Alternative names
- BTB (POZ) domain containing 17 antibody
- BTB/POZ domain-containing protein 17 antibody
- btbd17 antibody
see all
Images
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All lanes : Anti-BTBD17 antibody [EPR9593(B)] (ab138507) at 1/1000 dilution
Lane 1 : Human colon cancer lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human bladder lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTBD17 antibody [EPR9593(B)] (ab138507)
Immunohistochemical analysis of paraffin embedded Human kidney clear cell carcinoma tissue labelling BTBD17 with ab138507 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTBD17 antibody [EPR9593(B)] (ab138507)
Immunohistochemical analysis of paraffin embedded Human liver tissue labelling BTBD17 with ab138507 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (1)
ab138507 has been referenced in 1 publication.
- Kobayashi J et al. Extracellular a-synuclein enters dopaminergic cells by modulating flotillin-1-assisted dopamine transporter endocytosis. FASEB J 33:10240-10256 (2019). PubMed: 31211923