Key features and details
- Rabbit polyclonal to BTBD2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BTBD2 antibody
DescriptionRabbit polyclonal to BTBD2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human BTBD2 aa 321-525.
Database link: Q9BX70
- WB: HEK-293T and HeLa whole cell lysate. IHC-P: Human kidney tissue. ICC/IF: MCF-7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 56 kDa.|
|ICC/IF||1/50 - 1/200.|
RelevanceThe C-terminus of BTBD2 binds topoisomerase I. The N-terminus contains a proline-rich region and a BTB/POZ domain (broad-complex, Tramtrack and bric a brac/Pox virus and Zinc finger), both of which are typically involved in protein-protein interactions. Subcellularly, the protein localizes to cytoplasmic bodies. There are two named isoforms.
Cellular localizationCytoplasm, P-body. Note: Cytoplasmic bodies.
- BTB (POZ) domain containing 2 antibody
- BTB/POZ domain containing protein 2 antibody
- BTBD 2 antibody
All lanes : Anti-BTBD2 antibody (ab237737) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 56 kDa
Paraffin-embedded human kidney tissue stained for BTBD2 using ab237736 at 1/50 dilution in immunohistochemical analysis.
MCF7 (human breast adenocarcinoma cell line) cells stained for BTBD2 (Green) using ab237737 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237737 has not yet been referenced specifically in any publications.