Overview

  • Product name

    Anti-BTBD2 antibody
  • Description

    Rabbit polyclonal to BTBD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human BTBD2 aa 321-525.
    Database link: Q9BX70

  • Positive control

    • WB: HEK-293T and HeLa whole cell lysate. IHC-P: Human kidney tissue. ICC/IF: MCF-7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity greater than 95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 56 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Relevance

    The C-terminus of BTBD2 binds topoisomerase I. The N-terminus contains a proline-rich region and a BTB/POZ domain (broad-complex, Tramtrack and bric a brac/Pox virus and Zinc finger), both of which are typically involved in protein-protein interactions. Subcellularly, the protein localizes to cytoplasmic bodies. There are two named isoforms.
  • Cellular localization

    Cytoplasm, P-body. Note: Cytoplasmic bodies.
  • Database links

  • Alternative names

    • BTB (POZ) domain containing 2 antibody
    • BTB/POZ domain containing protein 2 antibody
    • BTBD 2 antibody

Images

  • All lanes : Anti-BTBD2 antibody (ab237737) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 56 kDa

  • Paraffin-embedded human kidney tissue stained for BTBD2 using ab237736 at 1/50 dilution in immunohistochemical analysis.

  • MCF7 (human breast adenocarcinoma cell line) cells stained for BTBD2 (Green) using ab237737 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

References

ab237737 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab237737.
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