Key features and details
- Rabbit polyclonal to BTF
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-BTF antibody
See all BTF primary antibodies
DescriptionRabbit polyclonal to BTF
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
Recombinant fragment within Human BTF (internal sequence). The exact sequence is proprietary.
Database link: Q9NYF8
- WB: K562 whole cell and nuclear extract lysate. ICC/IF: MCF7 cells. IHC: Mouse cervix and rat brain tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 106 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionDeath-promoting transcriptional repressor.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Bcl 2 associated transcription factor antibody
- Bcl-2-associated transcription factor 1 antibody
- BCL2 associated transcription factor 1 antibody
All lanes : Anti-BTF antibody (ab226830) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : K562 nuclear extract lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 106 kDa
4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for BTF using ab226830 at 1/500 dilution in ICC/IF.
Paraffin-embedded mouse cervix tissue stained for PFA using ab226830 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat brain tissue stained for PFA using ab226830 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226830 has not yet been referenced specifically in any publications.