Overview

  • Product name

    Anti-BTF antibody - C-terminal
    See all BTF primary antibodies
  • Description

    Rabbit polyclonal to BTF - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment within Human BTF (C terminal). The exact sequence is proprietary.
    Database link: Q9NYF8

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab226796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 106 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-BTF antibody - C-terminal (ab226796) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 106 kDa



    5% SDS-PAGE

  • 4% paraformaldehyde-fixed, A431 (human epidermoid carcinoma cell line) cells stained for BTF (green) using ab226796 at 1/500 dilution in ICC/IF.

    The cytoskeleton is stained with phalloidin (red).

References

ab226796 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226796.
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