Key features and details
- Rabbit polyclonal to BTF3
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BTF3 antibody
See all BTF3 primary antibodies
DescriptionRabbit polyclonal to BTF3
Tested applicationsSuitable for: IHC-P, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Bat
Synthetic peptide corresponding to a region between amino acids 35-70 of Human BTF3 (NP_001032726.1).
- HeLa whole cell lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab113974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 10 µg/mg of lysate.|
FunctionGeneral transcription factor. BTF3 can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
Sequence similaritiesBelongs to the NAC-beta family.
Contains 1 NAC-A/B (NAC-alpha/beta) domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Basic transcription factor 3 antibody
- BETA NAC antibody
- BTF 3 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and human prostate carcinoma (right) tissues labelling BTF3 with ab113974 at 1/1000 (1µg/ml). Detection: DAB.
Detection of BTF3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab113974 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-BTF3 antibody which recognizes a upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Predicted band size : 22 kDa.
ab113974 has not yet been referenced specifically in any publications.