Overview

  • Product name

    Anti-BTG1 antibody [EPR8274(2)]
    See all BTG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8274(2)] to BTG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the Human BTG1 protein (P62324).

  • Positive control

    • WB: Human fetal kidney, SH-SY5Y, NIH/3T3, and MCF-7 cell lysates. FC: SH-SY5Y cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-BTG1 antibody [EPR8274(2)] (ab151740) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : NIH/3T3 cell lysate
      Lane 4 : MCF-7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 19 kDa

    • Flow cytometric analysis of permeabilized SH-SY5Y cells labeling BTG1 with ab151740 antibody at 1/10 dilution (Red), or with a rabbit IgG (Green, negative).

    References

    This product has been referenced in:

    • Qian Y  et al. The treatment effects and the underlying mechanism of B cell translocation gene 1 on the oncogenesis of brain glioma. J Cell Biochem N/A:N/A (2019). Read more (PubMed: 30916818) »
    • Lee AS  et al. eIF3 targets cell-proliferation messenger RNAs for translational activation or repression. Nature 522:111-4 (2015). WB . Read more (PubMed: 25849773) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    mouse embryonic stem cells (J1)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Apr 10 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up