Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPRZJU-21] to BTG4
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse
Product nameAnti-BTG4 antibody [EPRZJU-21]
See all BTG4 primary antibodies
DescriptionRabbit monoclonal [EPRZJU-21] to BTG4
SpecificityWe have not been able to detect the endogenous mouse BTG4 with ab206914.
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse
Recombinant full length protein within Mouse BTG4 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: O70552
- WB: HeLa whole cell lysate transfected with mouse BTG4 with GFP-tag. ICC/IF: HeLa cells transfected with GFP-BTG4. IP: 293T transfected with mouse BTG4 with HA-tag whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab206914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
We have not been able to detect the endogenous mouse BTG4 with ab206914.
FunctionShows marked antiproliferative activity, being able to induce G(1) arrest.
Tissue specificityHighly expressed in testis and in olfactory epithelium.
Sequence similaritiesBelongs to the BTG family.
- Information by UniProt
- B cell translocation gene 4 antibody
- BTG family member 4 antibody
- Btg4 antibody
All lanes : Anti-BTG4 antibody [EPRZJU-21] (ab206914) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate transfected with empty vector (vector control)
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate lysate transfected with mouse BTG4 with GFP-tag
Lane 3 : Human ovary lysate
Lane 4 : Mouse ovary lysate
Lane 5 : Mouse testis lysate
Lane 6 : Rat ovary lysate
Lane 7 : Rat testis lysate
Lane 8 : Mouse P0 olfactory epithelium lysate
Lane 9 : Rat P0 olfactory epithelium lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BTG4 with ab206914 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cell transfected with GFP-BTG4.
The nuclear counterstain is DAPI (blue).
BTG4 was immunoprecipitated from 1mg of 293T (Human epithelial cell line from embryonic kidney) transfected with mouse BTG4 with HA-tag whole cell lysate with ab206914 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using ab206914 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: 293T transfected with mouse BTG4 with HA-tag whole cell lysate, 10µg (Input).
Lane 2: ab206914 IP in 293T transfected with mouse BTG4 with HA-tag whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab206914 in 293T transfected with mouse BTG4 with HA-tag whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
ab206914 has been referenced in 1 publication.
- Yu C et al. BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing factor in oocytes. Nat Struct Mol Biol N/A:N/A (2016). PubMed: 27065194