Recombinant
RabMAb

Recombinant Anti-BTG4 antibody [EPRZJU-21] (ab206914)

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Overview

  • Product name

    Anti-BTG4 antibody [EPRZJU-21]
    See all BTG4 primary antibodies

  • Description

    Rabbit monoclonal [EPRZJU-21] to BTG4

  • Host species

    Rabbit

  • Specificity

    We have not been able to detect the endogenous mouse BTG4 with ab206914.

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant full length protein within Mouse BTG4 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: O70552

  • Positive control

    • WB: HeLa whole cell lysate transfected with mouse BTG4 with GFP-tag. ICC/IF: HeLa cells transfected with GFP-BTG4. IP: 293T transfected with mouse BTG4 with HA-tag whole cell lysate.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPRZJU-21

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

We have not been able to detect the endogenous mouse BTG4 with ab206914.
ICC/IF 1/1000.
IP 1/50.

Target

  • Function

    Shows marked antiproliferative activity, being able to induce G(1) arrest.

  • Tissue specificity

    Highly expressed in testis and in olfactory epithelium.

  • Sequence similarities

    Belongs to the BTG family.
  • Information by UniProt

  • Database links

    • Alternative names

      • B cell translocation gene 4 antibody
      • BTG family member 4 antibody
      • Btg4 antibody
      • BTG4_HUMAN antibody
      • MGC33003 antibody
      • PC3B antibody
      • Protein BTG4 antibody
      • Protein PC3b antibody
      see all

    Images

    • Western blot - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
      Western blot - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
      All lanes : Anti-BTG4 antibody [EPRZJU-21] (ab206914) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate transfected with empty vector (vector control)
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate lysate transfected with mouse BTG4 with GFP-tag
      Lane 3 : Human ovary lysate
      Lane 4 : Mouse ovary lysate
      Lane 5 : Mouse testis lysate
      Lane 6 : Rat ovary lysate
      Lane 7 : Rat testis lysate
      Lane 8 : Mouse P0 olfactory epithelium lysate
      Lane 9 : Rat P0 olfactory epithelium lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 29 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
      Immunocytochemistry/ Immunofluorescence - Anti-BTG4 antibody [EPRZJU-21] (ab206914)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BTG4 with ab206914 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

      Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cell transfected with GFP-BTG4.

      The nuclear counterstain is DAPI (blue).

    • Immunoprecipitation - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
      Immunoprecipitation - Anti-BTG4 antibody [EPRZJU-21] (ab206914)

      BTG4 was immunoprecipitated from 1mg of 293T (Human epithelial cell line from embryonic kidney) transfected with mouse BTG4 with HA-tag whole cell lysate with ab206914 at 1/50 dilution.

      Western blot was performed from the immunoprecipitate using ab206914 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: 293T transfected with mouse BTG4 with HA-tag whole cell lysate, 10µg (Input).

      Lane 2: ab206914 IP in 293T transfected with mouse BTG4 with HA-tag whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab206914 in 293T transfected with mouse BTG4 with HA-tag whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

    References

    This product has been referenced in:

    • Yu C  et al. BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing factor in oocytes. Nat Struct Mol Biol N/A:N/A (2016). Read more (PubMed: 27065194) »
    See 1 Publication for this product

    Publishing research using ab206914? Please let us know so that we can cite the reference in this datasheet.

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