Product nameAnti-BTG4 antibody [EPRZJU-21]
See all BTG4 primary antibodies
DescriptionRabbit monoclonal [EPRZJU-21] to BTG4
SpecificityWe have not been able to detect the endogenous mouse BTG4 with ab206914.
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse
Recombinant full length protein within Mouse BTG4 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: O70552
- WB: HeLa whole cell lysate transfected with mouse BTG4 with GFP-tag. ICC/IF: HeLa cells transfected with GFP-BTG4. IP: 293T transfected with mouse BTG4 with HA-tag whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab206914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
We have not been able to detect the endogenous mouse BTG4 with ab206914.
FunctionShows marked antiproliferative activity, being able to induce G(1) arrest.
Tissue specificityHighly expressed in testis and in olfactory epithelium.
Sequence similaritiesBelongs to the BTG family.
- Information by UniProt
- B cell translocation gene 4 antibody
- BTG family member 4 antibody
- Btg4 antibody
All lanes : Anti-BTG4 antibody [EPRZJU-21] (ab206914) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate transfected with empty vector (vector control)
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate lysate transfected with mouse BTG4 with GFP-tag
Lane 3 : Human ovary lysate
Lane 4 : Mouse ovary lysate
Lane 5 : Mouse testis lysate
Lane 6 : Rat ovary lysate
Lane 7 : Rat testis lysate
Lane 8 : Mouse P0 olfactory epithelium lysate
Lane 9 : Rat P0 olfactory epithelium lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BTG4 with ab206914 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cell transfected with GFP-BTG4.
The nuclear counterstain is DAPI (blue).
BTG4 was immunoprecipitated from 1mg of 293T (Human epithelial cell line from embryonic kidney) transfected with mouse BTG4 with HA-tag whole cell lysate with ab206914 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using ab206914 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: 293T transfected with mouse BTG4 with HA-tag whole cell lysate, 10µg (Input).
Lane 2: ab206914 IP in 293T transfected with mouse BTG4 with HA-tag whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab206914 in 293T transfected with mouse BTG4 with HA-tag whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.