RecombinantRabMAb

Recombinant Anti-BTG4 antibody [EPRZJU-21] (ab206914)

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Western blot - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
  • Immunocytochemistry/ Immunofluorescence - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
  • Immunoprecipitation - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
  • Anti-BTG4 antibody [EPRZJU-21] (ab206914)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPRZJU-21] to BTG4
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-BTG4 antibody [EPRZJU-21]
    See all BTG4 primary antibodies

  • Description

    Rabbit monoclonal [EPRZJU-21] to BTG4

  • Host species

    Rabbit

  • Specificity

    We have not been able to detect the endogenous mouse BTG4 with ab206914.

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant full length protein within Mouse BTG4 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: O70552

  • Positive control

    • WB: HeLa whole cell lysate transfected with mouse BTG4 with GFP-tag. ICC/IF: HeLa cells transfected with GFP-BTG4. IP: 293T transfected with mouse BTG4 with HA-tag whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPRZJU-21

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

We have not been able to detect the endogenous mouse BTG4 with ab206914.
ICC/IF 1/1000.
IP 1/50.

Target

  • Function

    Shows marked antiproliferative activity, being able to induce G(1) arrest.

  • Tissue specificity

    Highly expressed in testis and in olfactory epithelium.

  • Sequence similarities

    Belongs to the BTG family.
  • Information by UniProt

  • Database links

    • Alternative names

      • B cell translocation gene 4 antibody
      • BTG family member 4 antibody
      • Btg4 antibody
      • BTG4_HUMAN antibody
      • MGC33003 antibody
      • PC3B antibody
      • Protein BTG4 antibody
      • Protein PC3b antibody
      see all

    Images

    • Western blot - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
      Western blot - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
      All lanes : Anti-BTG4 antibody [EPRZJU-21] (ab206914) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate transfected with empty vector (vector control)
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate lysate transfected with mouse BTG4 with GFP-tag
      Lane 3 : Human ovary lysate
      Lane 4 : Mouse ovary lysate
      Lane 5 : Mouse testis lysate
      Lane 6 : Rat ovary lysate
      Lane 7 : Rat testis lysate
      Lane 8 : Mouse P0 olfactory epithelium lysate
      Lane 9 : Rat P0 olfactory epithelium lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 29 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
      Immunocytochemistry/ Immunofluorescence - Anti-BTG4 antibody [EPRZJU-21] (ab206914)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BTG4 with ab206914 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

      Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cell transfected with GFP-BTG4.

      The nuclear counterstain is DAPI (blue).

    • Immunoprecipitation - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
      Immunoprecipitation - Anti-BTG4 antibody [EPRZJU-21] (ab206914)

      BTG4 was immunoprecipitated from 1mg of 293T (Human epithelial cell line from embryonic kidney) transfected with mouse BTG4 with HA-tag whole cell lysate with ab206914 at 1/50 dilution.

      Western blot was performed from the immunoprecipitate using ab206914 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: 293T transfected with mouse BTG4 with HA-tag whole cell lysate, 10µg (Input).

      Lane 2: ab206914 IP in 293T transfected with mouse BTG4 with HA-tag whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab206914 in 293T transfected with mouse BTG4 with HA-tag whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

    • Anti-BTG4 antibody [EPRZJU-21] (ab206914)
      Anti-BTG4 antibody [EPRZJU-21] (ab206914)

    References (1)

    Publishing research using ab206914? Please let us know so that we can cite the reference in this datasheet.

    ab206914 has been referenced in 1 publication.

    • Yu C  et al. BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing factor in oocytes. Nat Struct Mol Biol N/A:N/A (2016). PubMed: 27065194

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