Key features and details
- Rabbit polyclonal to BTK
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-BTK antibody
See all BTK primary antibodies
DescriptionRabbit polyclonal to BTK
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide, corresponding to 16 N terminal amino acids near the amino terminus of Human BTK.
- U937 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesBTK Antibody is affinity chromatography purified via peptide column.
Our Abpromise guarantee covers the use of ab25971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 73 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionPlays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
Involvement in diseaseDefects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
modificationsAutophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
Cellular localizationCytoplasm. Membrane. Nucleus.
- Information by UniProt
- Agammaglobulinaemia tyrosine kinase antibody
- AGMX 1 antibody
- AGMX1 antibody
Lane 1 : Anti-BTK antibody (ab25971) at 0.5 µg/ml
Lane 2 : Anti-BTK antibody (ab25971) at 1 µg/ml
Lane 3 : Anti-BTK antibody (ab25971) at 2 µg/ml
All lanes : for analysis of U937 cell lysate (15ug lysate/lane)
Predicted band size: 73 kDa
Immunohistochemistry of BTK in mouse brain tissue with BTK antibody at 5 µg/ml.
ab52971 at 10µg/ml staining BTK in Daudi cells by ICC/IF
Immunofluorescence of BTK in Daudi cells using ab25971 at 10 ug/ml.
ab25971 has not yet been referenced specifically in any publications.