Recombinant Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)
![Western blot - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)](https://www.abcam.com/ps/products/40/ab40770/Images/ab40770-266841-anti-btk-phospho-y551-antibody-ep267y-western-blot.jpg "Western blot - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP267Y] to BTK (phospho Y551)
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BTK (phospho Y551) antibody [EP267Y]
See all BTK primary antibodies -
Description
Rabbit monoclonal [EP267Y] to BTK (phospho Y551) -
Host species
Rabbit -
Specificity
Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.
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Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Sample: Ramos treated with Pervanadate whole cell lysates. ICC/IF: Ramos cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP267Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/500.
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| WB |
1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
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| Notes |
|---|
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ICC/IF
1/500. |
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WB
1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
Target
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Function
Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes. -
Involvement in disease
Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD). -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Post-translational
modificationsAutophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein. -
Cellular localization
Cytoplasm. Membrane. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 695 Human
- Omim: 300300 Human
- SwissProt: Q06187 Human
- Unigene: 159494 Human
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Alternative names
- Agammaglobulinaemia tyrosine kinase antibody
- AGMX 1 antibody
- AGMX1 antibody
see all
Images
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Western blot - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)All lanes : Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770) at 1/5000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma) treated with Pervanadate whole cell lysates
The treatment protocols were not recorded.
Lane 2 : Untreated Ramos (human Burkitt's lymphoma) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 30 secondsBlocking/Diluting buffer 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)](https://www.abcam.com/ps/products/40/ab40770/Images/ab40770-3-anti-btk-phospho-y551-antibody-ep267y-immunocytochemistry-ramos-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) treated with pervanadate (1mM 30min) cells labeling BTK with purified ab40770 at 1/500 dilution (2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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![Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)](https://www.abcam.com/ps/products/40/ab40770/Images/ab40770-4-benefits-of-recombinant-antibodies.png)
Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab40770 has been referenced in 5 publications.
- Li WF et al. HIV-1 Nef dimers short-circuit immune receptor signaling by activating Tec-family kinases at the host cell membrane. J Biol Chem 295:5163-5174 (2020). PubMed: 32144207
- Mo ZQ et al. Molecular characterization and function analysis of grouper (Epinephelus coioides) Bruton's tyrosine kinase BTK. Fish Shellfish Immunol 77:91-99 (2018). WB . PubMed: 29567142
- Rushworth SA et al. Identification of Bruton's tyrosine kinase as a therapeutic target in acute myeloid leukemia. Blood 123:1229-38 (2014). Human . PubMed: 24307721
- Daniel JL et al. Cbl-b is a novel physiologic regulator of glycoprotein VI-dependent platelet activation. J Biol Chem 285:17282-91 (2010). PubMed: 20400514
- Grasis JA et al. In vivo significance of ITK-SLP-76 interaction in cytokine production. Mol Cell Biol 30:3596-609 (2010). PubMed: 20457812