Recombinant Anti-BTN1A1 antibody [EPR14173] (ab180605)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14173] to BTN1A1
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-BTN1A1 antibody [EPR14173]
See all BTN1A1 primary antibodies -
Description
Rabbit monoclonal [EPR14173] to BTN1A1 -
Host species
Rabbit -
Tested Applications & Species
Application Species IP HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human milk.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14173 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC |
Human
|
IHC-P |
Human
|
IP |
Human
|
WB |
Human
|
All applications |
Mouse
Rat
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 62, 66 kDa (predicted molecular weight: 59 kDa).
|
|
IP |
1/50.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 62, 66 kDa (predicted molecular weight: 59 kDa). |
IP
1/50. |
Target
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Function
May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane (By similarity). Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 B30.2/SPRY domain.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 696 Human
- Entrez Gene: 12231 Mouse
- Entrez Gene: 306956 Rat
- Omim: 601610 Human
- SwissProt: Q13410 Human
- SwissProt: Q62556 Mouse
- Unigene: 153058 Human
- Unigene: 1420 Mouse
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Alternative names
- BT antibody
- BT1A1_HUMAN antibody
- BTN antibody
see all
Images
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Anti-BTN1A1 antibody [EPR14173] (ab180605) at 1/50000 dilution + Human milk at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 59 kDa
Observed band size: 62,66 kDa why is the actual band size different from the predicted?66 kDa: full length
62 kDa: fragment -
Western blot analysis of Human milk immunoprecipitated with ab180605 at 1/50 dilution (Lane 1). Lane2 : Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution.
Protocols
Datasheets and documents
Certificate of Compliance
References (1)
ab180605 has been referenced in 1 publication.
- Sun X et al. High expression of cell death-inducing DFFA-like effector a (CIDEA) promotes milk fat content in dairy cows with clinical ketosis. J Dairy Sci N/A:N/A (2018). PubMed: 30594378