RecombinantRabMAb

Recombinant Anti-BTN1A1 antibody [EPR14173] (ab180605)

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Western blot - Anti-BTN1A1 antibody [EPR14173] (ab180605)
  • Immunoprecipitation - Anti-BTN1A1 antibody [EPR14173] (ab180605)
  • Anti-BTN1A1 antibody [EPR14173] (ab180605)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14173] to BTN1A1
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-BTN1A1 antibody [EPR14173]
    See all BTN1A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14173] to BTN1A1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human milk.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14173

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
IHC-P
Human
IP
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 62, 66 kDa (predicted molecular weight: 59 kDa).
IP
1/50.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 62, 66 kDa (predicted molecular weight: 59 kDa).
IP
1/50.

Target

  • Function

    May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane (By similarity). Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 B30.2/SPRY domain.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.

  • Post-translational
    modifications

    N-glycosylated.

  • Cellular localization

    Membrane. Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • BT antibody
      • BT1A1_HUMAN antibody
      • BTN antibody
      • BTN1 antibody
      • Btn1a1 antibody
      • butyrophilin antibody
      • Butyrophilin subfamily 1 member A1 antibody
      • butyrophilin, subfamily 1, member A1 antibody
      see all

    Images

    • Western blot - Anti-BTN1A1 antibody [EPR14173] (ab180605)
      Western blot - Anti-BTN1A1 antibody [EPR14173] (ab180605)
      Anti-BTN1A1 antibody [EPR14173] (ab180605) at 1/50000 dilution + Human milk at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 59 kDa
      Observed band size: 62,66 kDa
      why is the actual band size different from the predicted?



      66 kDa: full length
      62 kDa: fragment

    • Immunoprecipitation - Anti-BTN1A1 antibody [EPR14173] (ab180605)
      Immunoprecipitation - Anti-BTN1A1 antibody [EPR14173] (ab180605)

      Western blot analysis of Human milk  immunoprecipitated with ab180605 at 1/50 dilution (Lane 1). Lane2 : Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution.

    • Anti-BTN1A1 antibody [EPR14173] (ab180605)
      Anti-BTN1A1 antibody [EPR14173] (ab180605)

    References (1)

    Publishing research using ab180605? Please let us know so that we can cite the reference in this datasheet.

    ab180605 has been referenced in 1 publication.

    • Sun X  et al. High expression of cell death-inducing DFFA-like effector a (CIDEA) promotes milk fat content in dairy cows with clinical ketosis. J Dairy Sci N/A:N/A (2018). PubMed: 30594378

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