Recombinant Anti-BTN1A1 antibody [EPR14173] (ab180605)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-BTN1A1 antibody [EPR14173]
See all BTN1A1 primary antibodies -
Description
Rabbit monoclonal [EPR14173] to BTN1A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human BTN1A1 aa 50-150. The exact sequence is proprietary.
Database link: Q13410 -
Positive control
- Human milk.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14173 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab180605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 62, 66 kDa (predicted molecular weight: 59 kDa). | |
| IP | 1/50. |
Target
-
Function
May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane (By similarity). Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 B30.2/SPRY domain.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 696 Human
- Entrez Gene: 12231 Mouse
- Entrez Gene: 306956 Rat
- Omim: 601610 Human
- SwissProt: Q13410 Human
- SwissProt: Q62556 Mouse
- Unigene: 153058 Human
- Unigene: 1420 Mouse
-
Alternative names
- BT antibody
- BT1A1_HUMAN antibody
- BTN antibody
see all
Images
-
![Western blot - Anti-BTN1A1 antibody [EPR14173] (ab180605)](https://www.abcam.com/ps/products/180/ab180605/Images/ab180605-211981-anti-btn1a1-antibody-epr14173-western-blot.jpg)
Western blot - Anti-BTN1A1 antibody [EPR14173] (ab180605)Anti-BTN1A1 antibody [EPR14173] (ab180605) at 1/50000 dilution + Human milk at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 59 kDa
Observed band size: 62,66 kDa why is the actual band size different from the predicted?66 kDa: full length
62 kDa: fragment -
![Immunoprecipitation - Anti-BTN1A1 antibody [EPR14173] (ab180605)](https://www.abcam.com/ps/products/180/ab180605/Images/ab180605-211982-anti-btn1a1-antibody-epr14173-immunoprecipitation.jpg)
Immunoprecipitation - Anti-BTN1A1 antibody [EPR14173] (ab180605)Western blot analysis of Human milk immunoprecipitated with ab180605 at 1/50 dilution (Lane 1). Lane2 : Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution.
Datasheets and documents
References
ab180605 has not yet been referenced specifically in any publications.
