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RecombinantRabMAb

Recombinant Anti-BTN1A1 antibody [EPR14173] (ab180605)

Submit a review Submit a question References (1)
Western blot - Anti-BTN1A1 antibody [EPR14173] (ab180605)
  • Immunoprecipitation - Anti-BTN1A1 antibody [EPR14173] (ab180605)
  • Anti-BTN1A1 antibody [EPR14173] (ab180605)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14173] to BTN1A1
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-BTN1A1 antibody [EPR14173]
    See all BTN1A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14173] to BTN1A1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human milk.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14173

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 62, 66 kDa (predicted molecular weight: 59 kDa).
IP
1/50.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 62, 66 kDa (predicted molecular weight: 59 kDa).
IP
1/50.

Target

  • Function

    May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane (By similarity). Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 B30.2/SPRY domain.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.

  • Post-translational
    modifications

    N-glycosylated.

  • Cellular localization

    Membrane. Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • BT antibody
      • BT1A1_HUMAN antibody
      • BTN antibody
      • BTN1 antibody
      • Btn1a1 antibody
      • butyrophilin antibody
      • Butyrophilin subfamily 1 member A1 antibody
      • butyrophilin, subfamily 1, member A1 antibody
      see all

    Images

    • Western blot - Anti-BTN1A1 antibody [EPR14173] (ab180605)
      Western blot - Anti-BTN1A1 antibody [EPR14173] (ab180605)
      Anti-BTN1A1 antibody [EPR14173] (ab180605) at 1/50000 dilution + Human milk at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 59 kDa
      Observed band size: 62,66 kDa why is the actual band size different from the predicted?



      66 kDa: full length
      62 kDa: fragment

    • Immunoprecipitation - Anti-BTN1A1 antibody [EPR14173] (ab180605)
      Immunoprecipitation - Anti-BTN1A1 antibody [EPR14173] (ab180605)

      Western blot analysis of Human milk  immunoprecipitated with ab180605 at 1/50 dilution (Lane 1). Lane2 : Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution.

    • Anti-BTN1A1 antibody [EPR14173] (ab180605)
      Anti-BTN1A1 antibody [EPR14173] (ab180605)

    References (1)

    Publishing research using ab180605? Please let us know so that we can cite the reference in this datasheet.

    ab180605 has been referenced in 1 publication.

    • Sun X  et al. High expression of cell death-inducing DFFA-like effector a (CIDEA) promotes milk fat content in dairy cows with clinical ketosis. J Dairy Sci N/A:N/A (2018). PubMed: 30594378

    Customer reviews and Q&As

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