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Rabbit polyclonal to BTN2A1
antigen sequence: ILSGEKEFER ETREIALKEL EKERVQKEEE LQVKEKLQEE LRWRRTFLHA VDVVLD, corresponding to amino acids 278-333 of Human BTN2A1.
- Human skeletal muscle tissue; RT 4 and U 251 MG lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas. Moderate expression was seen in lung, liver and kidney.
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 B30.2/SPRY domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Information by UniProt
- BK14H9.1 antibody
- BT2.1 antibody
- BT2A1_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-BTN2A1 antibody (ab121593)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane. Recommended concentration of ab121593 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN2A1 antibody (ab121593)
ab121593 at 1/50 dilution staining BTN2A1 in Paraffin-embedded Human Skeletal tissue by Immunohistochemistry.
Western blot - Anti-BTN2A1 antibody (ab121593)
All lanes : Anti-BTN2A1 antibody (ab121593) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"