Anti-BTN2A2/BTF2 antibody [6C7D2] (ab233763)
Key features and details
- Mouse monoclonal [6C7D2] to BTN2A2/BTF2
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-BTN2A2/BTF2 antibody [6C7D2]
See all BTN2A2/BTF2 primary antibodies -
Description
Mouse monoclonal [6C7D2] to BTN2A2/BTF2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human BTN2A2/BTF2 aa 57-237. (Expressed in E.coli).
Database link: Q8WVV5 -
Positive control
- WB: Recombinant human BTN2A2/BTF2 protein; BTN2A2/BTF2 (AA: extra 57-237)-hIgGFc transfected HEK-293 cell lysate. Flow: HeLa cells. IHC-P: Cervical cancer and bladder cancer tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6C7D2 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
|
|
IHC-P |
1/200 - 1/1000.
|
|
Flow Cyt |
1/200 - 1/400.
|
Notes |
---|
WB
1/500 - 1/2000. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. |
Target
-
Tissue specificity
Highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas. Moderate expression was seen in lung, liver and kidney. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 B30.2/SPRY domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 10385 Human
- Omim: 613591 Human
- SwissProt: Q8WVV5 Human
- Unigene: 373938 Human
-
Alternative names
- BT2.2 antibody
- BT2A2_HUMAN antibody
- BTF2 antibody
see all
Images
-
Paraffin-embedded human bladder cancer tissue stained for BTN2A2/BTF2 using ab233763 at 1/200 dilution in immunohistochemical analysis. DAB staining.
-
Paraffin-embedded human cervical cancer tissue stained for BTN2A2/BTF2 using ab233763 at 1/200 dilution in immunohistochemical analysis. DAB staining.
-
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling BTN2A2/BTF2 using ab233763 at 1/200 dilution (green) compared to a negative control (red).
-
All lanes : Anti-BTN2A2/BTF2 antibody [6C7D2] (ab233763) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : BTN2A2/BTF2 (AA: extra 57-237)-hIgGFc transfected HEK-293 cell lysate
Developed using the ECL technique. -
Anti-BTN2A2/BTF2 antibody [6C7D2] (ab233763) at 1/500 dilution + Recombinant human GCSF receptor (AA: extra 25-187) protein
Expected MW is 46.4 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab233763 has not yet been referenced specifically in any publications.