Overview

  • Product name

    Anti-BTN2A2/BTF2 antibody [6C7D2]
    See all BTN2A2/BTF2 primary antibodies
  • Description

    Mouse monoclonal [6C7D2] to BTN2A2/BTF2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human BTN2A2/BTF2 aa 57-237. (Expressed in E.coli).
    Database link: Q8WVV5

  • Positive control

    • WB: Recombinant human BTN2A2/BTF2 protein; BTN2A2/BTF2 (AA: extra 57-237)-hIgGFc transfected HEK-293 cell lysate. Flow: HeLa cells. IHC-P: Cervical cancer and bladder cancer tissue.
  • General notes

     This product was previously labelled as BTN2A2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab233763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

Target

  • Tissue specificity

    Highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas. Moderate expression was seen in lung, liver and kidney.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 B30.2/SPRY domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BT2.2 antibody
    • BT2A2_HUMAN antibody
    • BTF2 antibody
    • BTN2A2 antibody
    • butyrophilin 2 antibody
    • Butyrophilin subfamily 2 member A2 antibody
    see all

Images

  • Paraffin-embedded human bladder cancer tissue stained for BTN2A2/BTF2 using ab233763 at 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Paraffin-embedded human cervical cancer tissue stained for BTN2A2/BTF2 using ab233763 at 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling BTN2A2/BTF2 using ab233763 at 1/200 dilution (green) compared to a negative control (red).

  • All lanes : Anti-BTN2A2/BTF2 antibody [6C7D2] (ab233763) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : BTN2A2/BTF2 (AA: extra 57-237)-hIgGFc transfected HEK-293 cell lysate.
  • Anti-BTN2A2/BTF2 antibody [6C7D2] (ab233763) at 1/500 dilution + Recombinant human BTN2A2/BTF2 protein


    Expected MW is 46.4 kDa.

References

ab233763 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233763.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up