Key features and details
- Mouse monoclonal [6C7D2] to BTN2A2/BTF2
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-BTN2A2/BTF2 antibody [6C7D2]
See all BTN2A2/BTF2 primary antibodies
DescriptionMouse monoclonal [6C7D2] to BTN2A2/BTF2
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human BTN2A2/BTF2 aa 57-237. (Expressed in E.coli).
Database link: Q8WVV5
- WB: Recombinant human BTN2A2/BTF2 protein; BTN2A2/BTF2 (AA: extra 57-237)-hIgGFc transfected HEK-293 cell lysate. Flow: HeLa cells. IHC-P: Cervical cancer and bladder cancer tissue.
This product was previously labelled as BTN2A2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab233763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000.|
|IHC-P||1/200 - 1/1000.|
|Flow Cyt||1/200 - 1/400.|
Tissue specificityHighly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas. Moderate expression was seen in lung, liver and kidney.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 B30.2/SPRY domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- BT2.2 antibody
- BT2A2_HUMAN antibody
- BTF2 antibody
Paraffin-embedded human bladder cancer tissue stained for BTN2A2/BTF2 using ab233763 at 1/200 dilution in immunohistochemical analysis. DAB staining.
Paraffin-embedded human cervical cancer tissue stained for BTN2A2/BTF2 using ab233763 at 1/200 dilution in immunohistochemical analysis. DAB staining.
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling BTN2A2/BTF2 using ab233763 at 1/200 dilution (green) compared to a negative control (red).
All lanes : Anti-BTN2A2/BTF2 antibody [6C7D2] (ab233763) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : BTN2A2/BTF2 (AA: extra 57-237)-hIgGFc transfected HEK-293 cell lysate.
Anti-BTN2A2/BTF2 antibody [6C7D2] (ab233763) at 1/500 dilution + Recombinant human BTN2A2/BTF2 protein
Expected MW is 46.4 kDa.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233763 has not yet been referenced specifically in any publications.