Overview

  • Product name

    Anti-BTN3A2 antibody [OTI1A6]
    See all BTN3A2 primary antibodies
  • Description

    Mouse monoclonal [OTI1A6] to BTN3A2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human BTN3A2. Produced in HEK293T cells (NP_008978).
    Database link: P78410

  • Positive control

    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRY BTN3A2. IHC-P: Human breast, colon, kidney, lung, ovary and endometrium tissues. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY BTN3A2 and HT29 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant by affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    OTI1A6
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 36 kDa.
IHC-P 1/50.
ICC/IF 1/100.

Target

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BT3.2 antibody
    • BT3.3 antibody
    • BT3A2_HUMAN antibody
    • BTF4 antibody
    • BTN3A 2 antibody
    • BTN3A2 antibody
    • Butyrophilin protein antibody
    • Butyrophilin subfamily 3 member A2 antibody
    • Butyrophilin, subfamily 3, member A2 antibody
    • CD277 antibody
    • FLJ40011 antibody
    see all

Images

  • COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY BTN3A2 stained for BTN3A2 (Green) using ab118051 at 1/100 dilution in ICC/IF.

     

  • All lanes : Anti-BTN3A2 antibody [OTI1A6] (ab118051) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY BTN3A2 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 36 kDa

  • Paraffin-embedded Human Breast tissue stained for BTN3A2 using ab118051 at 1/50 dilution in immunohistochemical analysis.

  • Paraffin-embedded Human colon tissue stained for BTN3A2 using ab118051 at 1/50 dilution in immunohistochemical analysis.

  • Paraffin-embedded Human kidney tissue stained for BTN3A2 using ab118051 at 1/50 dilution in immunohistochemical analysis.

  • Paraffin-embedded Human ovary tissue stained for BTN3A2 using ab118051 at 1/50 dilution in immunohistochemical analysis.

  • Paraffin-embedded Human lung tissue stained for BTN3A2 using ab118051 at 1/50 dilution in immunohistochemical analysis.

  • Paraffin-embedded Human endometrium tissue stained for BTN3A2 using ab118051 at 1/50 dilution in immunohistochemical analysis.

  • HT-29 (Human colorectal adenocarcinoma cell line) cells transiently transfected by pCMV6-ENTRY BTN3A2 stained for BTN3A2 (Green) using ab118051 at 1/100 dilution in ICC/IF.

     

References

ab118051 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab118051.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up