Key features and details
- Rabbit polyclonal to BTNL2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BTNL2 antibody
See all BTNL2 primary antibodies
DescriptionRabbit polyclonal to BTNL2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human BTNL2 aa 26-202.
Database link: Q9UIR0
- WB: HEK-293 and K562 whole cell lysates; mouse brain lysate. IHC-P: Human testis and pancreatic tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notes>955 pure
Our Abpromise guarantee covers the use of ab223053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 50.4 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionNegative regulator of T cell proliferation.
Tissue specificityExpressed in brain, heart, kidney, liver, pancreas, ovary, leukocyte, small intestine, testis and thymus.
Involvement in diseaseGenetic variation in BTNL2 is a cause of susceptibility to sarcoidosis type 2 (SS2) [MIM:612387]. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Note=A nucleotide transition affecting a splice donor site results in the use of an alternative splice site and the production of isoform 3. Individuals expressing isoform 3 have a higher risk for sarcoidosis.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 3 Ig-like V-type (immunoglobulin-like) domains.
Cellular localizationMembrane. Isoform 2 is present in the nuclear, vesicle and plasma membranes, isoform 3 is found in cytoplasmic vesicle structures and is not membrane bound.
- Information by UniProt
- BTL-II antibody
- Btnl2 antibody
- BTNL2_HUMAN antibody
All lanes : Anti-BTNL2 antibody (ab223053) at 1/2000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : Mouse brain lysate
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 50.4 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Paraffin embedded sections of human testis tissue were stained for BTNL2 with ab223053 at 1/100 dilution in immunohistochemical analysis.
Paraffin embedded sections of human pancreas tissue were stained for BTNL2 with ab223053 at 1/100 dilution in immunohistochemical analysis.
ab223053 has not yet been referenced specifically in any publications.