Overview

  • Product name

  • Description

    Rabbit polyclonal to BTNL2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human BTNL2 aa 26-202.
    Database link: Q9UIR0

  • Positive control

    • WB: HEK-293 and K562 whole cell lysates; mouse brain lysate. IHC-P: Human testis and pancreatic tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    >955 pure
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 50.4 kDa).
IHC-P 1/20 - 1/200.

Target

  • Function

    Negative regulator of T cell proliferation.
  • Tissue specificity

    Expressed in brain, heart, kidney, liver, pancreas, ovary, leukocyte, small intestine, testis and thymus.
  • Involvement in disease

    Genetic variation in BTNL2 is a cause of susceptibility to sarcoidosis type 2 (SS2) [MIM:612387]. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Note=A nucleotide transition affecting a splice donor site results in the use of an alternative splice site and the production of isoform 3. Individuals expressing isoform 3 have a higher risk for sarcoidosis.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 3 Ig-like V-type (immunoglobulin-like) domains.
  • Cellular localization

    Membrane. Isoform 2 is present in the nuclear, vesicle and plasma membranes, isoform 3 is found in cytoplasmic vesicle structures and is not membrane bound.
  • Information by UniProt
  • Database links

  • Alternative names

    • BTL-II antibody
    • Btnl2 antibody
    • BTNL2_HUMAN antibody
    • Butyrophilin like 2 (MHC class II associated) antibody
    • Butyrophilin-like protein 2 antibody
    • DAAP-282D22.2 antibody
    • HSBLMHC1 antibody
    • SS2 antibody
    see all

Images

  • All lanes : Anti-BTNL2 antibody (ab223053) at 1/2000 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
    Lane 3 : Mouse brain lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 50.4 kDa
    Observed band size: 51 kDa
    why is the actual band size different from the predicted?

  • Paraffin embedded sections of human testis tissue were stained for BTNL2 with ab223053 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin embedded sections of human pancreas tissue were stained for BTNL2 with ab223053 at 1/100 dilution in immunohistochemical analysis.

References

ab223053 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab223053.
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