Recombinant
RabMAb

Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)

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Overview

  • Product name
    Anti-BTNL3 antibody [EPR8411(2)(B)]
  • Description
    Rabbit monoclonal [EPR8411(2)(B)] to BTNL3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BTNL3 aa 300-400. The exact sequence is proprietary.
    Database link: Q6UXE8

  • Positive control
    • Human small intestine, Jurkat, Caco2 and K562 lysates. K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR8411(2)(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Tissue specificity
      Expressed in small intestine, colon, testis, spleen, and leukocyte.
    • Sequence similarities
      Belongs to the immunoglobulin superfamily. BTN/MOG family.
      Contains 1 B30.2/SPRY domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
      • Alternative names
        • BTNL3 antibody
        • BTNL3_HUMAN antibody
        • BTNLR antibody
        • Butyrophilin like protein 3 antibody
        • Butyrophilin like receptor antibody
        • Butyrophilin-like protein 3 antibody
        • Butyrophilin-like receptor antibody
        • COLF4100 antibody
        see all

      Images

      • Flow Cytometry - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
        Flow Cytometry - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)

        Flow cytometric analysis of permeabilized K562 cells labeling BTNL3 with ab177473 at 1/10 dilution (red)  or a rabbit IgG (negative) (green).

      • Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
        Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
        All lanes : Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473) at 1/1000 dilution

        Lane 1 : Human small intestine lysate
        Lane 2 : Jurkat lysate
        Lane 3 : Caco2 lysate
        Lane 4 : K562 lysate

        Lysates/proteins at 10 µg per lane.

        Developed using the ECL technique.

        Predicted band size: 52 kDa

      References

      ab177473 has not yet been referenced specifically in any publications.

      Publishing research using ab177473? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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