RecombinantRabMAb

Recombinant Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8411(2)(B)] to BTNL3
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-BTNL3 antibody [EPR8411(2)(B)]
    See all BTNL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8411(2)(B)] to BTNL3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human BTNL3 aa 300-400. The exact sequence is proprietary.
    Database link: Q6UXE8

  • Positive control

    • Human small intestine, Jurkat, Caco2 and K562 lysates. K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8411(2)(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Tissue specificity

      Expressed in small intestine, colon, testis, spleen, and leukocyte.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. BTN/MOG family.
      Contains 1 B30.2/SPRY domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • BTNL3 antibody
        • BTNL3_HUMAN antibody
        • BTNLR antibody
        • Butyrophilin like protein 3 antibody
        • Butyrophilin like receptor antibody
        • Butyrophilin-like protein 3 antibody
        • Butyrophilin-like receptor antibody
        • COLF4100 antibody
        see all

      Images

      • Flow Cytometry - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
        Flow Cytometry - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)

        Flow cytometric analysis of permeabilized K562 cells labeling BTNL3 with ab177473 at 1/10 dilution (red)  or a rabbit IgG (negative) (green).

      • Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
        Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
        All lanes : Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473) at 1/1000 dilution

        Lane 1 : Human small intestine lysate
        Lane 2 : Jurkat lysate
        Lane 3 : Caco2 lysate
        Lane 4 : K562 lysate

        Lysates/proteins at 10 µg per lane.

        Developed using the ECL technique.

        Predicted band size: 52 kDa

      • Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
        Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)

      References (1)

      Publishing research using ab177473? Please let us know so that we can cite the reference in this datasheet.

      ab177473 has been referenced in 1 publication.

      • Ramello MC  et al. An immunoproteomic approach to characterize the CAR interactome and signalosome. Sci Signal 12:N/A (2019). PubMed: 30755478

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