Recombinant Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-BTNL3 antibody [EPR8411(2)(B)]
See all BTNL3 primary antibodies -
Description
Rabbit monoclonal [EPR8411(2)(B)] to BTNL3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BTNL3 aa 300-400. The exact sequence is proprietary.
Database link: Q6UXE8 -
Positive control
- Human small intestine, Jurkat, Caco2 and K562 lysates. K562 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8411(2)(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab177473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 52 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Tissue specificity
Expressed in small intestine, colon, testis, spleen, and leukocyte. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 B30.2/SPRY domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10917 Human
- Omim: 606192 Human
- SwissProt: Q6UXE8 Human
- Unigene: 225949 Human
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Alternative names
- BTNL3 antibody
- BTNL3_HUMAN antibody
- BTNLR antibody
see all
Images
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![Flow Cytometry - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)](https://www.abcam.com/ps/products/177/ab177473/Images/ab177473-206195-anti-btnl3-antibody-epr84112b-flow-cytometry.jpg)
Flow Cytometry - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)Flow cytometric analysis of permeabilized K562 cells labeling BTNL3 with ab177473 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)](https://www.abcam.com/ps/products/177/ab177473/Images/ab177473-206194-anti-btnl3-antibody-epr84112b-western-blot.jpg)
Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)All lanes : Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473) at 1/1000 dilution
Lane 1 : Human small intestine lysate
Lane 2 : Jurkat lysate
Lane 3 : Caco2 lysate
Lane 4 : K562 lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 52 kDa
Datasheets and documents
References
ab177473 has not yet been referenced specifically in any publications.
