Key features and details
- Rabbit polyclonal to Bub3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Bub3 antibody
See all Bub3 primary antibodies
DescriptionRabbit polyclonal to Bub3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Orangutan
Synthetic peptide within Human Bub3 aa 1-50. The exact sequence is proprietary.
Database link: O43684
- WB: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|WB||1/5000 - 1/15000.|
FunctionHas a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Sequence similaritiesBelongs to the WD repeat BUB3 family.
Contains 5 WD repeats.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
- Information by UniProt
- Aa2-050 antibody
- AU019800 antibody
- AU021329 antibody
All lanes : Anti-Bub3 antibody (ab264121) at 0.066 µg/ml
Lane 1 : Mock treated HeLa whole cell lysate
Lane 2 : HeLa cell lysate treated with Nocodazole
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 30 seconds
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Bub3 using ab264121 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264121 has not yet been referenced specifically in any publications.