Product nameAnti-Bub3 antibody
See all Bub3 primary antibodies
DescriptionRabbit polyclonal to Bub3
Tested applicationsSuitable for: WB, EMSAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Xenopus laevis, Zebrafish
Synthetic peptide derived from residues 300 to the C-terminus of Human Bub3.
- HeLa Nuclear Extract;
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab4180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).|
|EMSA||Use at an assay dependent concentration. PubMed: 22210889|
FunctionHas a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Sequence similaritiesBelongs to the WD repeat BUB3 family.
Contains 5 WD repeats.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
- Information by UniProt
- Aa2-050 antibody
- AU019800 antibody
- AU021329 antibody
Anti-Bub3 antibody (ab4180) at 1/500 dilution + HeLa Nuclear Extract at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Western blot of ab4180 on 20ug HeLa Nuclear Extract at 1/500.
Secondary ab: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000 - 1 min exposure)
This product has been referenced in:
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). Read more (PubMed: 30377371) »
- Zhu S et al. A motif from Lys216 to Lys222 in human BUB3 protein is a nuclear localization signal and critical for BUB3 function in mitotic checkpoint. J Biol Chem 290:11282-92 (2015). Read more (PubMed: 25814666) »