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    bubr1-antibody-ab28194.pdf

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Cell Biology Cell Cycle Cell Division Spindle
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Anti-BubR1 antibody (ab28194)

  • Datasheet
  • SDS
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Immunoprecipitation - Anti-BubR1 antibody (ab28194)

    Key features and details

    • Sheep polyclonal to BubR1
    • Suitable for: IP
    • Reacts with: Mouse
    • Isotype: IgG

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    Recombinant human BubR1 protein (ab133149)
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    Overview

    • Product name

      Anti-BubR1 antibody
      See all BubR1 primary antibodies
    • Description

      Sheep polyclonal to BubR1
    • Host species

      Sheep
    • Tested applications

      Suitable for: IPmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Recombinant fragment, corresponding to amino acids 10-190 of Mouse BubR1

    • Positive control

      • IP: Mitotic NIH/3T3 cells.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.08% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Ammonium Sulphate Precipitation
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Cell Division
      • Spindle
      • Epigenetics and Nuclear Signaling
      • Cell cycle
      • Kinases/Phosphatases
      • Other
      • Cancer
      • Cell cycle
      • Cell division

    Associated products

    • Isotype control

      • Sheep IgG - Isotype Control (ab37385)
    • Positive Controls

      • NIH/3T3 whole cell lysate (ab7179)
    • Recombinant Protein

      • Recombinant human BubR1 protein (ab133149)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab28194 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP
    Notes
  • Application notes
    ICC: Use at an assay dependent dilution.
    Immunohistochemistry (PFA fixed): Use at an assay dependent dilution.
    IP: Use at a concentration of 1 - 10 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
    • Tissue specificity

      Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
    • Involvement in disease

      Note=Defects in BUB1B are associated with tumor formation.
      Defects in BUB1B are the cause of premature chromatid separation trait (PCS) [MIM:176430]. PCS consists of separate and splayed chromatids with discernible centromeres and involves all or most chromosomes of a metaphase. It is found in up to 2% of metaphases in cultured lymphocytes from approximately 40% of normal individuals. When PCS is present in 5% or more of cells, it is known as the heterozygous PCS trait and has no obvious phenotypic effect, although some have reported decreased fertility. Inheritance is autosomal dominant.
      Defects in BUB1B are the cause of mosaic variegated aneuploidy syndrome (MVA) [MIM:257300]. MVA is a severe autosomal recessive developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. The proportion of aneuploid cells varies but is usually more than 25% and is substantially greater than in normal individuals. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases. MVA is caused by biallelic mutations in the BUB1B gene.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.
      Contains 1 BUB1 N-terminal domain.
      Contains 1 protein kinase domain.
    • Domain

      The D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase.
      The BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.
    • Post-translational
      modifications

      Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.
      Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.
      Ubiquitinated. Degradated by the proteasome.
      Sumoylated by SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.
      Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.
    • Cellular localization

      Cytoplasm. Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5.
    • Target information above from: UniProt accession O60566 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12236 Mouse
      • SwissProt: Q9Z1S0 Mouse
      • Unigene: 29133 Mouse
      • Alternative names

        • Beta homolg of S. cerevisiae BUB 1 antibody
        • Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody
        • BUB 1B antibody
        • BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody
        • Bub1A antibody
        • BUB1B antibody
        • BUB1B_HUMAN antibody
        • BUB1beta antibody
        • BUBR1 antibody
        • Budding Uninhibited by Benzimidazoles 1 beta antibody
        • Budding uninhibited by benzimidazoles 1 homolog beta (yeast) antibody
        • hBUBR1 antibody
        • MAD3/BUB1 related protein kinase antibody
        • MAD3/BUB1-related protein kinase antibody
        • MAD3L antibody
        • Mitotic checkpoint gene BUB1B antibody
        • Mitotic checkpoint kinase MAD3L antibody
        • Mitotic checkpoint serine/threonine protein kinase BUB1 beta antibody
        • Mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody
        • MVA1 antibody
        • OTTHUMP00000160319 antibody
        • Protein SSK1 antibody
        • SSK 1 antibody
        • SSK1 antibody
        see all

      Images

      • Immunoprecipitation - Anti-BubR1 antibody (ab28194)
        Immunoprecipitation - Anti-BubR1 antibody (ab28194)

        Immunoprecipitation/ Western blot analysis using Bub1b-NT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow cytometry.

      Protocols

      • Immunoprecipitation protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab28194? Please let us know so that we can cite the reference in this datasheet.

      ab28194 has been referenced in 1 publication.

      • Qiao D  et al. Glypican-1 regulates anaphase promoting complex/cyclosome substrates and cell cycle progression in endothelial cells. Mol Biol Cell 19:2789-801 (2008). WB ; Mouse . PubMed: 18417614

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