Key features and details
- Rabbit polyclonal to BUD31
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-BUD31 antibody
DescriptionRabbit polyclonal to BUD31
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Zebrafish
- WB: Jurkat, Raji, NIH/3T3, K562, HepG2 and U-251 MG whole cell lysate; Mouse liver lysate. IHC-P: Human colon cancer and spleen tissues. ICC/IF: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 17 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
Sequence similaritiesBelongs to the BUD31 (G10) family.
- Information by UniProt
- Bud31 antibody
- BUD31 homolog (S. cerevisiae) antibody
- BUD31_HUMAN antibody
All lanes : Anti-BUD31 antibody (ab237493) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 7 : U-251 MG (human brain glioma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 17 kDa
Paraffin-embedded human colon cancer tissue stained for BUD31 using ab237493 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human spleen tissue stained for BUD31 using ab237493 at 1/100 dilution in immunohistochemical analysis.
MCF7 (human breast adenocarcinoma cell line) cells stained for BUD31 (green) using ab237493 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237493 has not yet been referenced specifically in any publications.