Anti-Butyrylcholinesterase antibody (ab154763)

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Overview

  • Product name
    Anti-Butyrylcholinesterase antibody
    See all Butyrylcholinesterase primary antibodies
  • Description
    Rabbit polyclonal to Butyrylcholinesterase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 59-297 of the Human Butyrylcholinesterase (UniProt ID: P06276).

  • Positive control
    • 293T, HeLa and Raji whole cell lysate; Human SAS xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab154763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
  • Tissue specificity
    Detected in blood plasma (at protein level). Present in most cells except erythrocytes.
  • Involvement in disease
    Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]. BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait.
  • Sequence similarities
    Belongs to the type-B carboxylesterase/lipase family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Acylcholine acylhydrolase antibody
    • BCHE antibody
    • Butyrylcholine esterase antibody
    • CHE1 antibody
    • CHE2 antibody
    • CHLE_HUMAN antibody
    • Choline esterase II antibody
    • Cholinesterase (serum) 2 antibody
    • Cholinesterase 1 antibody
    • Cholinesterase antibody
    • E1 antibody
    • Pseudocholinesterase antibody
    • Pseudocholinesterase E1 antibody
    see all

Images

  • Western blot - Anti-Butyrylcholinesterase antibody (ab154763)
    Western blot - Anti-Butyrylcholinesterase antibody (ab154763)
    Anti-Butyrylcholinesterase antibody (ab154763) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 68 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Butyrylcholinesterase antibody (ab154763)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Butyrylcholinesterase antibody (ab154763)
    Immunohistochemical analysis of paraffin embedded Human SAS xenograft tissue labeling Butyrylcholinesterase with ab154763 at 1/100 dilution.

References

ab154763 has not yet been referenced specifically in any publications.

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