Anti-Butyrylcholinesterase antibody (ab154763)
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Overview
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Product nameAnti-Butyrylcholinesterase antibody
See all Butyrylcholinesterase primary antibodies -
DescriptionRabbit polyclonal to Butyrylcholinesterase
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cat
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Immunogen
Recombinant fragment corresponding to a region within amino acids 59-297 of the Human Butyrylcholinesterase (UniProt ID: P06276).
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Positive control
- 293T, HeLa and Raji whole cell lysate; Human SAS xenograft tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 68 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionEsterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
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Tissue specificityDetected in blood plasma (at protein level). Present in most cells except erythrocytes.
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Involvement in diseaseDefects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]. BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait.
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Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 590 Human
- Entrez Gene: 12038 Mouse
- Entrez Gene: 65036 Rat
- Omim: 177400 Human
- SwissProt: P06276 Human
- SwissProt: Q03311 Mouse
- Unigene: 420483 Human
- Unigene: 250719 Mouse
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Alternative names
- Acylcholine acylhydrolase antibody
- BCHE antibody
- Butyrylcholine esterase antibody
see all
Images
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Western blot - Anti-Butyrylcholinesterase antibody (ab154763)Anti-Butyrylcholinesterase antibody (ab154763) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 68 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Butyrylcholinesterase antibody (ab154763)Immunohistochemical analysis of paraffin embedded Human SAS xenograft tissue labeling Butyrylcholinesterase with ab154763 at 1/100 dilution.
References
ab154763 has not yet been referenced specifically in any publications.
