Anti-Butyrylcholinesterase antibody (ab154763)

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Key features and details

  • Rabbit polyclonal to Butyrylcholinesterase
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Butyrylcholinesterase antibody
    See all Butyrylcholinesterase primary antibodies

  • Description

    Rabbit polyclonal to Butyrylcholinesterase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat

  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 59-297 of the Human Butyrylcholinesterase (UniProt ID: P06276).

  • Positive control

    • 293T, HeLa and Raji whole cell lysate; Human SAS xenograft tissue

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab154763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.

  • Tissue specificity

    Detected in blood plasma (at protein level). Present in most cells except erythrocytes.

  • Involvement in disease

    Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]. BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait.

  • Sequence similarities

    Belongs to the type-B carboxylesterase/lipase family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Acylcholine acylhydrolase antibody
    • BCHE antibody
    • Butyrylcholine esterase antibody
    • CHE1 antibody
    • CHE2 antibody
    • CHLE_HUMAN antibody
    • Choline esterase II antibody
    • Cholinesterase (serum) 2 antibody
    • Cholinesterase 1 antibody
    • Cholinesterase antibody
    • E1 antibody
    • Pseudocholinesterase antibody
    • Pseudocholinesterase E1 antibody
    see all

Images

  • Western blot - Anti-Butyrylcholinesterase antibody (ab154763)
    Western blot - Anti-Butyrylcholinesterase antibody (ab154763)
    Anti-Butyrylcholinesterase antibody (ab154763) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 68 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Butyrylcholinesterase antibody (ab154763)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Butyrylcholinesterase antibody (ab154763)
    Immunohistochemical analysis of paraffin embedded Human SAS xenograft tissue labeling Butyrylcholinesterase with ab154763 at 1/100 dilution.

References (0)

Publishing research using ab154763? Please let us know so that we can cite the reference in this datasheet.

ab154763 has not yet been referenced specifically in any publications.

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