Key features and details
- Rabbit polyclonal to Butyrylcholinesterase
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Butyrylcholinesterase antibody
See all Butyrylcholinesterase primary antibodies
DescriptionRabbit polyclonal to Butyrylcholinesterase
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow
Recombinant fragment corresponding to Human Butyrylcholinesterase aa 311-524.
GTPLSVNFGPTVDGDFLTDMPDILLELGQFKKTQILVGVNKDEGTAFLVY GAPGFSKDNNSIITRKEFQEGLKIFFPGVSEFGKESILFHYTDWVDDQRP ENYREALGDVVGDYNFICPALEFTKKFSEWGNNAFFYYFEHRSSKLPWPE WMGVMHGYEIEFVFGLPLERRDNYTKAEEILSRSIVKRWANFAKYGNPNE TQNNSTSWPVFKST
Database link: P06276
- WB: K562 whole cell lysate. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 68 kDa.|
FunctionEsterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Tissue specificityDetected in blood plasma (at protein level). Present in most cells except erythrocytes.
Involvement in diseaseDefects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]. BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait.
Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
- Information by UniProt
- Acylcholine acylhydrolase antibody
- BCHE antibody
- Butyrylcholine esterase antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Butyrylcholinesterase (Green) using ab236577 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Anti-Butyrylcholinesterase antibody (ab236577) at 1/500 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 68 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236577 has not yet been referenced specifically in any publications.