Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
Product nameButyrylcholinesterase Assay Kit
See all Butyrylcholinesterase kits
Butyrylcholinesterase Assay Kit (ab241010) is based on the ability of Butyrylcholinesterase (BChE) to hydrolyze substrate and produce thiocholine. Thiocholine reacts with 5, 5’-dithiobis (2-nitrobenzoic acid) (DTNB) and generates a yellow chromophore that can be quantified at 412 nm. The assay is simple, sensitive and can detect as low as 0.2 U/ml in variety of samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests BChE Assay Buffer 1 x 50ml BChE Substrate (in DMSO) 1 x 100µl Butyrylcholinesterase 1 vial DTNB 1 vial TNB Standard 1 vial
FunctionEsterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Tissue specificityDetected in blood plasma (at protein level). Present in most cells except erythrocytes.
Involvement in diseaseDefects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]. BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait.
Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
- Information by UniProt
- Acylcholine acylhydrolase
- Butyrylcholine esterase
ab241010 has not yet been referenced specifically in any publications.