Overview

  • Product name
  • Description
    Rabbit polyclonal to BVR
  • Host species
    Rabbit
  • Specificity
    ab19260 detects a 41-42 kDa protein in human samples and a 33 kDa protein in rat samples on SDS-PAGE immunoblots, corresponding to the apparent molecular weight of BVR.
  • Tested applications
    Suitable for: IHC-Fr, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human, Pig
  • Immunogen

    Recombinant full length protein corresponding to Rat BVR.

  • Positive control
    • recombinant rat BVR (Stressgen Biotechnologies) ICC: HeLa cells overexpressing BVR constructs IHC-P: rat kidney
  • General notes

    Protein previously labeled as Biliverdin Reductase.

Properties

Applications

Our Abpromise guarantee covers the use of ab19260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
ICC
  • Application notes
    ICC: 1/1000 - 1/5000.
    IHC-Fr: 1/1000 - 1/5000 (perfusion fixed cryosections) (See recommended protocol, amplification required).
    WB: 1/5000. Predicted molecular weight: 33 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor.
    • Tissue specificity
      Liver.
    • Pathway
      Porphyrin-containing compound metabolism; protoheme degradation.
    • Involvement in disease
      Hyperbiliverdinemia
    • Sequence similarities
      Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • BIEA_HUMAN antibody
      • Biliverdin IX alpha reductase antibody
      • Biliverdin reductase A antibody
      • Biliverdin-IX alpha-reductase antibody
      • BLVR A antibody
      • BLVR antibody
      • Blvra antibody
      • BVR A antibody
      • BVR antibody
      • BVRA antibody
      • Zinc metalloprotein antibody
      see all

    References

    This product has been referenced in:
    • Xing S  et al. Effect of the herbal formulation Shen-Zhi-Ling on an APP/PS1 mouse model of Alzheimer's disease by modulating the biliverdin reductase/heme oxygenase 1 system. Exp Ther Med 14:1961-1966 (2017). Read more (PubMed: 28962110) »
    • Wang T  et al. Proteomic modeling for HIV-1 infected microglia-astrocyte crosstalk. PLoS ONE 3:e2507 (2008). WB ; Mouse . Read more (PubMed: 18575609) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    As we discussed we have no information or references regarding the levels of biliverdin reductase in HepG2 cells therefore we cannot tell if the antibodies against this protein will work in this lysate. The positive control used for this antibody in Western blotting was recombinant rat biliverdin reductase (BVR) that was purchased from Stressgen Biotechnologies. I hope this information will help your customer,

    Read More

    Question
    Answer

    Thank you for your enquiry. We do not have a recommended positive control for this antiserum. However, following some literature searching I have found out that Biliverdin Reductase is a protein that is expressed in the liver and brain, albeit in subtly different forms. Please see: http://molpharm.aspetjournals.org/cgi/content/abstract/38/4/481 May I suggest that you use rat brain or liver lysates as positive controls if you are applying this antiserum in western blotting. We do sell a Rat brain lysate - ab7942. I hope this information helps, please do not hesitate to contact me should you require further details.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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